HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Slepova, T. V. Articles by Bonch-Osmolovskaya, E. A. Search for Related Content PubMed PubMed Citation Articles by Slepova, T. V. Articles by Bonch-Osmolovskaya, E. A. Agricola Articles by Slepova, T. V. Articles by Bonch-Osmolovskaya, E. A.

Carboxydothermus siderophilus sp. nov., a thermophilic, hydrogenogenic, carboxydotrophic, dissimilatory Fe(III)-reducing bacterium from a Kamchatka hot spring

¹ Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60 Let Oktyabrya 7/2, 117312 Moscow, Russia
² Bioengineering Center, Russian Academy of Sciences, Prospect 60 Let Oktyabrya 7/1, 117312 Moscow, Russia

Correspondence
Tatiana V. Slepova
slepysh{at}gmail.com

A novel anaerobic, thermophilic, Fe(III)-reducing, CO-utilizing bacterium, strain 1315^T, was isolated from a hot spring of Geyser Valley on the Kamchatka Peninsula. Cells of the new isolate were Gram-positive, short rods. Growth was observed at 52–70 °C, with an optimum at 65 °C, and at pH 5.5–8.5, with an optimum at pH 6.5–7.2. In the presence of Fe(III) or 9,10-anthraquinone 2,6-disulfonate (AQDS), the bacterium was capable of growth with CO and yeast extract (0.2 g l^–1); during growth under these conditions, strain 1315^T produced H₂ and CO₂ and Fe(II) or AQDSH₂, respectively. Strain 1315^T also grew by oxidation of yeast extract, glucose, xylose or lactate under a N₂ atmosphere, reducing Fe(III) or AQDS. Yeast extract (0.2 g l^–1) was required for growth. Isolate 1315^T grew exclusively with Fe(III) or AQDS as an electron acceptor. The generation time under optimal conditions with CO as growth substrate was 9.3 h. The G+C content of the DNA was 41.5±0.5 mol%. 16S rRNA gene sequence analysis placed the organism in the genus Carboxydothermus (97.8 % similarity with the closest relative). On the basis of physiological features and phylogenetic analysis, it is proposed that strain 1315^T should be assigned to a novel species, Carboxydothermus siderophilus sp. nov., with the type strain 1315^T (=VKPM 9905B^T =VKM B-2474^T =DSM 21278^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain 1315^T is EF542810.