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Int J Syst Evol Microbiol 59 (2009), 2904-2908; DOI  10.1099/ijs.0.009811-0
© 2009 International Union of Microbiological Societies

Methylophilus rhizosphaerae sp. nov., a restricted facultative methylotroph isolated from rice rhizosphere soil

Munusamy Madhaiyan1, Selvaraj Poonguzhali1, Soon-Wo Kwon2 and Tong-Min Sa1

1 Department of Agricultural Chemistry, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea
2 Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, RDA, Suwon 441-707, Republic of Korea

Correspondence
Tongmin Sa
tomsa{at}chungbuk.ac.kr

Three facultative methylotrophic bacterial strains, designated CBMB127T, CBMB145 and CBMB147, were isolated from the rhizosphere soil of rice and characterized. The strains produced indole-3-acetic acid and siderophores, had 1-aminocyclopropane-1-carboxylate deaminase activity and sulfur oxidation property and also methanol dehydrogenase. Phylogenetic analysis based on the 16S rRNA and methanol dehydrogenase (mxaF) gene sequences showed that Methylophilus methylotrophus was their close relative. The results of the phenotypic, phylogenetic and genotypic analyses showed that strains CBMB127T and CBMB145, with 99.4 % 16S rRNA gene sequence similarity and 99 % DNA–DNA hybridization, could be distinguished from recognized species of Methylophilus. Therefore strain CBMB127T and CBMB145 are considered to represent a novel species of Methylophilus, for which the name Methylophilus rhizosphaerae sp. nov. is proposed, with CBMB127T (=KACC 13099T=NCCB 100233T) as the type strain. Strain CBMB147 represents a novel strain of the species Methylophilus methylotrophus.

Abbreviations: ACCD, 1-aminocyclopropane-1-carboxylate deaminase; AMS, ammonium mineral salts; IAA, indole-3-acetic acid

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and mxaF gene sequences of strain CBMB127T are EU194887 and EU194904, respectively, and those for strain CBMB145 are EU194883 and EU194902 and those for CBMB147 are EU194892 and EU194905, respectively.

A neighbour-joining phylogenetic tree based on deduced amino acid sequences of mxaF products is available as supplementary material with the online version of this paper.






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