IJSEM Visit JGV Online
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
ijs.0.007336-0v1
59/11/2743    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Google Scholar
Right arrow Articles by Lee, Y.-J.
Right arrow Articles by Wiegel, J.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, Y.-J.
Right arrow Articles by Wiegel, J.
Agricola
Right arrow Articles by Lee, Y.-J.
Right arrow Articles by Wiegel, J.
Int J Syst Evol Microbiol 59 (2009), 2743-2746; DOI  10.1099/ijs.0.007336-0
© 2009 International Union of Microbiological Societies

Desulfosporosinus youngiae sp. nov., a spore-forming, sulfate-reducing bacterium isolated from a constructed wetland treating acid mine drainage

Yong-Jin Lee1,3, Christopher S. Romanek2,3 and Juergen Wiegel1

1 Department of Microbiology, University of Georgia, Athens, GA 30602, USA
2 Department of Geology, University of Georgia, Athens, GA 30602, USA
3 Savannah River Ecology Laboratory, Aiken, SC 29802, USA

Correspondence
Juergen Wiegel
jwiegel{at}uga.edu

Strain JW/YJL-B18T, a spore-forming, sulfate-reducing bacterium, was isolated from constructed wetland sediment. Cells were curved rods, 0.7–1.2 µm in diameter and 3–7 µm long. Despite being phylogenetically a member of the Gram-type-positive phylum Firmicutes, cells stained Gram-negative at all growth phases. Strain JW/YJL-B18T grew at 8–39 °C, with an optimum at 32–35 °C and no growth at 4 °C or below or at 42 °C or above. The pH25 °C range for growth was 5.7–8.2, with an optimum at pH25 °C 7.0–7.3, and no growth was detected at or below pH 5.2 or at or above pH 8.4. The salinity range for growth was 0–3 % (NaCl/KCl 9 : 1). Strain JW/YJL-B18T utilized as carbon and energy sources beef extract, yeast extract, formate, succinate, lactate, pyruvate, ethanol and toluene. Fumarate, sulfate, sulfite and thiosulfate were reduced in the presence of lactate. Arsenate (V) was not used as an electron acceptor. Strain JW/YJL-B18T showed no indication of growth under autotrophic conditions. The predominant cellular fatty acids were C16 : 1 and C16 : 0. The genomic DNA G+C content was 36.6 mol% (HPLC). 16S rRNA gene sequence analysis indicated that strain JW/YJL-B18T fell into the genus Desulfosporosinus, with Desulfosporosinus auripigmenti OREX-4T as its closest neighbour with a validly published name (97.9 % similarity). Based on molecular genetic evidence and physiological and biochemical characters including differences in the DNA G+C content, we propose to place strain JW/YJL-B18T (=DSM 17734T =ATCC BAA-1261T) as the type strain of a novel species, Desulfosporosinus youngiae sp. nov.

Abbreviations: SRB, sulfate-reducing bacteria

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JW/YJL-B18T is DQ117470.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2009 by the International Union of Microbiological Societies.