HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Figures and Tables All Versions of this Article: ijs.0.003046-0v1 59/11/2714    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Google Scholar Articles by Shashidhar, R. Articles by Bandekar, J. R. PubMed PubMed Citation Articles by Shashidhar, R. Articles by Bandekar, J. R. Agricola Articles by Shashidhar, R. Articles by Bandekar, J. R.

Deinococcus piscis sp. nov., a radiation-resistant bacterium isolated from a marine fish

Food Technology Division, Bhabha Atomic Research Centre, Mumbai 400 085, India

Correspondence
Jayant R. Bandekar
jrb{at}barc.gov.in

A radiation-resistant, Gram-stain-positive, non-motile, non-sporulating, aerobic, coccoid bacterium, strain 3ax^T, was isolated from a marine fish (black pomfret, Parastromateus niger), with radiation as a selective pressure. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 3ax^T exhibited highest similarity (97.9 %) with Deinococcus proteolyticus DSM 20540^T. The T_m for DNA–DNA hybridization of D. proteolyticus DSM 20540^T and strain 3ax^T was 15.3 °C, indicating that the novel strain was distinct from D. proteolyticus DSM 20540^T. The predominant respiratory quinone was MK-8. Strain 3ax^T could grow at 20–42 °C; the optimum temperature for growth was 35 °C. The strain grew well at pH 6–9, with optimum growth at pH 7. The cell wall contained ornithine. The major fatty acids were 16 : 0, 16 : 17c, 16 : 19c and 18 : 19c. Three phosphoglycolipids and one aminophospholipid were the major polar lipids. Based on the genotypic, phenotypic and chemotaxonomic characteristics, strain 3ax^T was significantly different from D. proteolyticus DSM 20540^T and, therefore, it was identified as representing a novel species of the genus Deinococcus, for which the name Deinococcus piscis sp. nov. is proposed. The type strain is 3ax^T (=MTCC9123^T =DSM 19767^T).

A 16S rRNA gene sequence-based UPGMA tree, detailed DNA–DNA hybridization results, polar lipid and fatty acid profiles and a comparison of properties of strain 3ax^T and related type strains are available as supplementary material with the online version of this paper.