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Tepidimicrobium xylanilyticum sp. nov., an anaerobic xylanolytic bacterium, and emended description of the genus Tepidimicrobium

¹ State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China
² Shanghai Key Laboratory of Bio-energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, PR China

Correspondence
Xiuzhu Dong
dongxz{at}sun.im.ac.cn

A novel, xylanolytic, anaerobic, moderately thermophilic bacterium, strain PML14^T, was isolated from the sludge of a thermophilic anaerobic digester treating municipal solid waste and sewage in Beijing, China. The strain was a Gram-positive, spore-forming and motile rod. Growth of the novel strain was observed at 25–67 °C (optimum 60 °C) and pH 5.8–9.3 (optimum pH 8.5). Strain PML14^T grew on a number of carbohydrates, including xylan, xylose, glucose and cellobiose, and a variety of proteinaceous compounds, including peptone, tryptone, Casamino acids, yeast extract, beef extract, casein hydrolysate, L-cysteine, L-serine, L-lysine, L-glycine, L-threonine, L-methionine and pyruvate. The fermentation products from glucose included acetate, ethanol, butyrate, hydrogen and carbon dioxide. Propionate was produced from xylan in addition to other compounds. Fe(III), 9,10-anthraquinone 2,6-disulfonate and thiosulfate were reduced with peptone as the electron donor. NH₃ was produced. Indole was not produced. Gelatin was not hydrolysed. The DNA G+C content of strain PML14^T was 36.2±0.8 mol% (T_m). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain PML14^T was related to the members of cluster XII of the clostridia, most closely to Tepidimicrobium ferriphilum SB91^T with 93.8 % 16S rRNA gene sequence similarity. On the basis of polyphasic evidence from this study, it is suggested that strain PML14^T (=CGMCC 1.5080^T=JCM 15035^T) represents a novel species of the genus Tepidimicrobium, for which the name Tepidimicrobium xylanilyticum sp. nov., is proposed. An emended description of the genus Tepidimicrobium is also provided.