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Dehalogenimonas lykanthroporepellens gen. nov., sp. nov., a reductively dehalogenating bacterium isolated from chlorinated solvent-contaminated groundwater

¹ Department of Civil and Environmental Engineering, Louisiana State University, Baton Rouge, LA 70803, USA
² Departamento de Zoologia and Centro de Neurociências, Universidade de Coimbra, 3004-517 Coimbra, Portugal
³ Departamento de Bioquímica and Centro de Neurociências, Universidade de Coimbra, 3001-401 Coimbra, Portugal
⁴ Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA

Correspondence
William M. Moe
moemwil{at}lsu.edu

Two recently reported bacterial strains that are able to reductively dehalogenate polychlorinated aliphatic alkanes, including 1,2,3-trichloropropane, 1,2-dichloropropane, 1,1,2,2-tetrachloroethane, 1,1,2-trichloroethane and 1,2-dichloroethane, were further characterized to clarify their taxonomic position. The two strains, designated BL-DC-8 and BL-DC-9^T, were mesophilic, non-spore-forming, non-motile, Gram-negative staining and strictly anaerobic. Cells were irregular cocci, 0.3–0.6 µm in diameter. The two strains were resistant to ampicillin and vancomycin. Hydrogen was utilized as an electron donor. The genomic DNA G+C content of strains BL-DC-8 and BL-DC-9^T was 54.0 and 53.8 mol%, respectively. The major cellular fatty acids were C_{18 : 1}9c, C_{16 : 1}9c, C_{16 : 0} and C_{14 : 0}. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the strains cluster within the phylum Chloroflexi, but are related only distantly to all recognized taxa in the phylum. Morphological, physiological and chemotaxonomic traits as well as phylogenetic analysis support the conclusion that these two strains represent a novel species of a new genus in the phylum Chloroflexi, for which the name Dehalogenimonas lykanthroporepellens gen. nov., sp. nov. is proposed. The type strain of Dehalogenimonas lykanthroporepellens is BL-DC-9^T (=ATCC BAA-1523^T =JCM 15061^T).

Scanning electron and transmission electron micrographs of cells of strains BL-DC-9^T and BL-DC-8 and phenotypic characteristics of type species of genera in the phylum Chloroflexi are available with the online version of this paper.

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				J. Yan, B. A. Rash, F. A. Rainey, and W. M. Moe Detection and Quantification of Dehalogenimonas and "Dehalococcoides" Populations via PCR-Based Protocols Targeting 16S rRNA Genes Appl. Envir. Microbiol., December 1, 2009; 75(23): 7560 - 7564. [Abstract] [Full Text] [PDF]