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Paenibacillus cellulositrophicus sp. nov., a cellulolytic bacterium from Thai soil

¹ Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
² Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand
³ Korean Collection for Type Cultures (KCTC), Biological Resource Center (BRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yusong, Daejeon 305-806, Republic of Korea

Correspondence
Somboon Tanasupawat
Somboon.T{at}chula.ac.th

A cellulolytic bacterium, strain P2-1^T, isolated from soil in Thailand, was characterized using a taxonomic approach based on phenotypic and chemotaxonomic characteristics and the 16S rRNA gene sequence. The novel strain was Gram-positive, facultatively anaerobic, spore-forming and rod-shaped. It contained meso-diaminopimelic as the diagnostic diamino acid in the cell-wall peptidoglycan. The DNA G+C content was 52.7 mol%. The major isoprenoid quinone was MK-7. Anteiso-C_{15 : 0} and iso-C_{16 : 0} were the dominant cellular fatty acids. Phylogenetic analyses using the 16S rRNA gene sequence showed that the novel strain was affiliated to the genus Paenibacillus. Strain P2-1^T was closely related to Paenibacillus cineris KCTC 3998^T, P. favisporus KCTC 3910^T and P. rhizosphaerae KCTC 13015^T with 96.3–96.5 % gene sequence similarity. DNA–DNA relatedness, physiological characteristics and some biochemical characteristics clearly distinguished strain P2-1^T from related species of the genus Paenibacillus. Therefore, strain P2-1^T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus cellulositrophicus sp. nov. is proposed. The type strain is P2-1^T (=KCTC 13135^T=PCU 305^T=TISTR 1888^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain P2-1^T is FJ178001.

A photograph showing the cellulase activity of colonies of strain P2-1^T on an agar plate and an extended phylogenetic tree are available as supplementary figures with the online version of this paper.