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Int J Syst Evol Microbiol 59 (2009), 2651-2655; DOI  10.1099/ijs.0.005090-0
© 2009 International Union of Microbiological Societies

Chitinilyticum litopenaei sp. nov., isolated from a freshwater shrimp pond, and emended description of the genus Chitinilyticum

Shu-Chen Chang1,2,{dagger}, Ming-Chang Wu2, Wen-Ming Chen3,{dagger}, Yung-Hsing Tsai3 and Tsong-Ming Lee4

1 Department of Hospitality Management, Tajen University, Pingtung, Taiwan, ROC
2 Department of Food Science, National Pingtung University of Science and Technology, Pingtung, Taiwan, ROC
3 Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung, Taiwan, ROC
4 Department of Food Science and Technology, Tajen University, Pingtung, Taiwan, ROC

Correspondence
Shu-Chen Chang
doris{at}mail.tajen.edu.tw

Strain c1T, originally isolated from surface water of a freshwater pond located in Pingtung (southern Taiwan) used for culture of Pacific white shrimp (Litopenaeus vannamei), was subjected to a polyphasic taxonomic analysis. The strain exhibited strong chitinolytic activity and was able to grow under aerobic and anaerobic conditions by utilizing chitin exclusively as the carbon, nitrogen and energy source. Phylogenetic analysis of the 16S rRNA gene sequence revealed a clear affiliation to the Betaproteobacteria, with the closest relatives being Chitinilyticum aquatile C14T and Chitinibacter tainanensis S1T, respectively showing 96.7 and 93.6 % 16S rRNA gene sequence similarity. The predominant fatty acids detected in cells of strain c1T were C16 : 0, C18 : 1{omega}7c and summed feature 3 (C16 : 1{omega}7c and/or iso-C15 : 0 2-OH). The G+C content of the genomic DNA was 62.2±1.0 mol%. On the basis of phylogenetic analysis, DNA–DNA hybridization data, physiological and biochemical characteristics and fatty acid compositions, the organism was shown to belong to the genus Chitinilyticum whilst representing a novel species within this genus, for which we propose the name Chitinilyticum litopenaei sp. nov. (type strain c1T =DSM 21440T =BCRC 17609T).

Abbreviations: GlcNAc, N-acetyl-β-D-glucosamine

{dagger}These authors contributed equally to this work.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain c1T is EU682455.

An HPLC profile of products of chitin degradation by strain c1T is available as supplementary material with the online version of this paper.






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