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Int J Syst Evol Microbiol 59 (2009), 2618-2623; DOI  10.1099/ijs.0.002691-0
© 2009 International Union of Microbiological Societies

Bhargavaea cecembensis gen. nov., sp. nov., isolated from the Chagos–Laccadive ridge system in the Indian Ocean

R. Manorama{dagger}, P. K. Pindi, G. S. N. Reddy{dagger} and S. Shivaji

Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India

Correspondence
S. Shivaji
shivas{at}ccmb.res.in

A novel Gram-positive, rod-shaped, non-motile, non-spore-forming bacterium, strain DSE10T, was isolated from a deep-sea sediment sample collected at a depth of 5904 m from the Chagos–Laccadive ridge system in the Indian Ocean. Cells of strain DSE10T were positive for catalase, oxidase, urease and lipase activities and contained iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and anteiso-C15 : 0 as the major fatty acids. The major respiratory quinones were MK-6 and MK-8 and the major lipids were phosphatidylglycerol and diphosphatidylglycerol. The cell-wall peptidoglycan contained diaminopimelic acid as the diagnostic diamino acid. A BLAST sequence similarity search based on 16S rRNA gene sequences indicated that the genera Planococcus, Planomicrobium, Bacillus and Geobacillus were the nearest phylogenetic neighbours to the novel isolate with gene sequence similarities ranging from 94.9 to 95.2 %. Phylogenetic analyses using neighbour-joining, minimum-evolution and maximum-parsimony methods indicated that strain DSE10T formed a deeply rooted lineage distinct from the clades represented by the genera Planococcus, Planomicrobium, Bacillus and Geobacillus. Further, strain DSE10T could be distinguished from the above-mentioned genera based on the presence of signature nucleotides G, A, C, T, C, A, G, C and T at positions 182, 444, 480, 492, 563, 931, 1253, 1300 and 1391, respectively, in the 16S rRNA gene sequence. Based on the phenotypic and phylogenetic characteristics determined in this study, strain DSE10T was assigned as the type species of a new genus, Bhargavaea gen. nov., as Bhargavaea cecembensis sp. nov. The type strain of Bhargavaea cecembensis gen. nov., sp. nov. is DSE10T (=LMG 24411T=JCM 14375T). The genomic DNA G+C content of strain DSE10T is 59.5±2.5 mol%.

Abbreviations: ME, minimum-evolution; NJ, neighbour-joining; TEM, transmission electron microscopy

{dagger}These authors contributed equally to this work.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Bhargavaea cecembensis gen. nov., sp. nov. DSE10T is AM286423.

Two additional phylogenetic trees based on 16S rRNA gene sequences and a table detailing the signature nucleotides of Bhargavaea gen. nov. are available with the online version of this paper.






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