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Thalassobacillus cyri sp. nov., a moderately halophilic Gram-positive bacterium from a hypersaline lake

¹ Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain
² Extremophiles Lab; Department of Microbiology, Faculty of Biology, College of Science, University of Tehran, Tehran, Iran
³ Department of Biology, Islamic Azad University, Science and Research Campus, Tehran, Iran

Correspondence
A. Ventosa
ventosa{at}us.es

A Gram-positive, moderately halophilic bacterium, designated strain HS286^T, was isolated from water of the hypersaline Lake Howz-Soltan in Iran. Cells were strictly aerobic, rod-shaped, motile and able to produce ellipsoidal endospores at a central-subterminal position in swollen sporangia. Isolate HS286^T grew in a complex medium supplemented with 1–15 % (w/v) NaCl, with optimum growth at 8.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that strain HS286^T was closely related to Thalassobacillus devorans G-19.1^T (99.4 % gene sequence similarity). The other closest species were Halobacillus yeomjeoni MSS-402^T (96.9 %) and other species of the genus Halobacillus (with 96.7–93.5 % similarity). Strain HS286^T had cell-wall peptidoglycan based on meso-diaminopimelic acid and MK-7 as the respiratory isoprenoid quinone. The major fatty acids were anteiso-C_{15 : 0} (43.8 %), iso-C_{16 : 0} (21.4 %), iso-C_{14 : 0} (9.4 %), anteiso-C_{17 : 0} (8.7 %) and iso-C_{15 : 0} (7.0 %) and the polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, two phospholipids and a glycolipid. The DNA G+C content was 43.0 mol%. All of these features confirmed the placement of isolate HS286^T within the genus Thalassobacillus. However DNA–DNA hybridization between strain HS286^T and the only recognized species of the genus Thalassobacillus, T. devorans G-19.1^T, was 27.3 %, showing unequivocally that the novel isolate constituted a new genospecies. Strain HS286^T could be clearly differentiated from T. devorans and other phylogenetic neighbours on the basis of several phenotypic, genotypic and chemotaxonomic features. Therefore, strain HS286^T constitutes a novel species, for which the name Thalassobacillus cyri sp. nov. is proposed. The type strain is HS286^T (=CCM 7597^T=JCM 15722^T).