HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Material All Versions of this Article: ijs.0.008870-0v1 59/10/2415    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Google Scholar Articles by Guo, X. Articles by Jiang, C.-Y. PubMed PubMed Citation Articles by Guo, X. Articles by Jiang, C.-Y. Agricola Articles by Guo, X. Articles by Jiang, C.-Y.

Alicyclobacillus aeris sp. nov., a novel ferrous- and sulfur-oxidizing bacterium isolated from a copper mine

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China

Correspondence
Cheng-Ying Jiang
jiangcy{at}sun.im.ac.cn

A novel mesophilic, acidophilic, endospore-forming bacterium, designated strain ZJ-6^T, was isolated from Zi-Jin copper mine in Inner Mongolia, China. Cells of strain ZJ-6^T were rod-shaped, stained Gram-positive or were Gram-variable, and grew aerobically at 25–35 °C (optimum, 30 °C) and pH 2.0–6.0 (optimum, pH 3.5). 16S rRNA gene sequence analysis showed that strain ZJ-6^T was related phylogenetically to members of the genus Alicyclobacillus, with 16S rRNA gene sequence similarities of 89.5–94.2 %. Cells contained MK-7 as the major quinone and the DNA G+C content was 51.2 mol%. Strain ZJ-6^T possessed a number of phenotypic characteristics that differentiated it from recognized Alicyclobacillus species, including its growth temperature, assimilation of various carbon sources, production of acids from a range of compounds, and the ability to grow chemoautotrophically using ferrous iron, elemental sulfur and tetrathionate as electron donors. The predominant cellular fatty acids of strain ZJ-6^T were anteiso-C_{15 : 0} (67.1 %), iso-C_{16 : 0} (7.7 %) and anteiso-C_{17 : 0} (7.4 %); -alicyclic fatty acids were not found. On the basis of these results, it is concluded that strain ZJ-6^T represents a novel species within the genus Alicyclobacillus, for which the name Alicyclobacillus aeris sp. nov. is proposed; the type strain is ZJ-6^T (=CGMCC 1.7072^T=NBRC 104953^T).

Phylogenetic trees based on 16S rRNA gene sequences constructed using the minimum-evolution and maximum-parsimony methods and a table showing the cellular fatty acid compositions of strain ZJ-6^T and related strains are available as supplementary material with the online version of this paper.