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Glaciecola lipolytica sp. nov., isolated from seawater near Tianjin city, China

Li-Ping Chen^1,2,, Hai-Ying Xu^1,2,, Song-Zhe Fu^1,2, Hong-Xia Fan^1,2, Ying-Hao Liu¹, Shuang-Jiang Liu¹ and Zhi-Pei Liu¹

¹ State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China
² Graduate University of Chinese Academy of Sciences, Beijing 100039, PR China

Correspondence
Zhi-Pei Liu
liuzhp{at}sun.im.ac.cn

A Gram-negative, non-spore-forming, rod-shaped bacterium, designated strain E3^T, which was motile with a polar flagellum, was isolated from coastal surface seawater near Tianjin city, China. The taxonomy of strain E3^T was studied by phenotypic, chemotaxonomic and phylogenetic methods. Strain E3^T forms non-pigmented colonies with a butyrous–mucoid consistency on marine 2216 agar. It grows optimally at 30 °C, in the presence of 2.0 % (w/v) NaCl and at pH 7.6. Strain E3^T is positive for catalase and oxidase and hydrolysis of lipid and reduces nitrate to N₂ gas. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain E3^T belongs to the Gammaproteobacteria and is grouped in the genus Glaciecola, with highest sequence similarity to the type strains of Glaciecola chathamensis (94.6 %), G. agarilytica (94.4 %) and G. mesophila (94.4 %). Strain E3^T contained summed feature 3 (C_{16 : 1}7c and/or iso-C_{15 : 0} 2-OH, 34.5 %), C_{18 : 1}7c (17.3 %) and C_{16 : 0} (17 %) as the major fatty acids and phosphatidylethanolamine and phosphatidylglycerol as the main phospholipids. The DNA G+C content was 40.8 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain E3^T is considered to represent a novel species of the genus Glaciecola, for which the name Glaciecola lipolytica sp. nov. is proposed. The type strain is E3^T (=CGMCC 1.7001^T =JCM 15139^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain E3^T is EU183316.

Transmission electron micrographs, 2D TLC of polar lipids and a comparison of fatty acid profiles are available as supplementary material with the online version of this paper.