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Int J Syst Evol Microbiol 59 (2009), 22-27; DOI  10.1099/ijs.0.001693-0
© 2009 International Union of Microbiological Societies

Methylobacterium phyllosphaerae sp. nov., a pink-pigmented, facultative methylotroph from the phyllosphere of rice

Munusamy Madhaiyan1, Selvaraj Poonguzhali1, Soon-Wo Kwon2 and Tong-Min Sa1

1 Department of Agricultural Chemistry, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea
2 Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Republic of Korea

Correspondence
Tong-Min Sa
tomsa{at}chungbuk.ac.kr

A pink-pigmented, aerobic, facultatively methylotrophic bacterial strain, CBMB27T, isolated from leaf tissues of rice (Oryza sativa L. ‘Dong-Jin’), was analysed using a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Methylobacterium oryzae, Methylobacterium fujisawaense and Methylobacterium mesophilicum; strain CBMB27T showed sequence similarities of 98.3, 98.5 and 97.3 %, respectively, to the type strains of these three species. DNA–DNA hybridization experiments revealed low levels (<38 %) of DNA–DNA relatedness between strain CBMB27T and its closest relatives. The sequence of the 1-aminocyclopropane-1-carboxylate deaminase gene (acdS) in strain CBMB27T differed from those of close relatives. The major fatty acid of the isolate was C18 : 1{omega}7c and the G+C content of the genomic DNA was 66.8 mol%. Based on the results of 16S rRNA gene sequence analysis, DNA–DNA hybridization, and physiological and biochemical characterization, which enabled the isolate to be differentiated from all recognized species of the genus Methylobacterium, it was concluded that strain CBMB27T represents a novel species in the genus Methylobacterium for which the name Methylobacterium phyllosphaerae sp. nov. is proposed (type strain CBMB27T =LMG 24361T =KACC 11716T =DSM 19779T).

Abbreviations: ACCD, 1-aminocyclopropane-1-carboxylate deaminase

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, mxaF and acdS gene sequences of strain CBMB27T are EF126746, EF562496 and EU430438, respectively.

API ZYM reactions of strain CBMB27T and related strains are available as supplementary material with the online version of this paper.






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