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Int J Syst Evol Microbiol 59 (2009), 186-194; DOI  10.1099/ijs.0.65830-0
© 2009 International Union of Microbiological Societies

Candida tumulicola sp. nov. and Candida takamatsuzukensis sp. nov., novel yeast species assignable to the Candida membranifaciens clade, isolated from the stone chamber of the Takamatsu-zuka tumulus

Yuka Nagatsuka1, Tomohiko Kiyuna1, Rika Kigawa2, Chie Sano2, Sadatoshi Miura2 and Junta Sugiyama3

1 NCIMB Group, TechnoSuruga Laboratory Co., Ltd, 330 Nagasaki, Shimizu-ku, Shizuoka-shi, Shizuoka 424-0065, Japan
2 Independent Administrative Institution, National Research Institute for Cultural Properties, Tokyo, 13-43 Ueno Park, Taito-ku, Tokyo 110-8713, Japan
3 Tokyo Office, TechnoSuruga Laboratory Co., Ltd, Shinko Music Plaza Bldg 5F-N, 2-1 Kandaogawa-machi, Chiyoda-ku, Tokyo 101-0052, Japan

Correspondence
Junta Sugiyama
jsugiyam{at}tecsrg.co.jp

During a survey of the mycobiota in the stone chamber of the Takamatsu-zuka tumulus in the village of Asuka, Nara Prefecture, Japan, we isolated 19 yeast strains assigned to the genus Candida from various samples, taken mainly from mouldy spots where the colour of the murals had changed to black, white or another tone, and from viscous gels (biofilms) on plaster walls. The 26S rDNA D1/D2 domain sequence-based phylogeny clearly indicates two groups of isolates. Polyphasic characterization, including morphological, physiological and chemotaxonomic characteristics, and sequence analysis of the 26S rDNA D1/D2 domain and ITS regions suggest that each group is assignable to one of two novel species within the Candida membranifaciens clade. Proposed herein are the names Candida tumulicola sp. nov. (originally T6517-9-5T; holotype JCM 15403T; isotypes CBS 10917T, NBRC 104392T) and Candida takamatsuzukensis sp. nov. (originally T4922-1-1T; holotype JCM 15410T; isotypes CBS 10916T, NBRC 104391T). The 26S rDNA D1/D2 domain sequence divergence indicates that C. tumulicola differs from Candida friedrichii NBRC 10277T, the type strain of the nearest species, in 15 nucleotides (3 %), whereas C. takamatsuzukensis differs from Candida insectorum NBRC 10283T and Pichia mexicana NBRC 10544T, the type strains of the nearest species, in 20 nucleotides (4 %). Both novel species are also clearly distinguishable from the species closest to them by various physiological characteristics.

Abbreviations: ITS, internal transcribed spacer

The GenBank/EMBL/DDBJ accession numbers for the combined ITS1 region, 5.8S rDNA, ITS2 region and 26S rDNA D1/D2 sequences determined in this study are AB365456–AB365477, as listed in Table 1.

A maximum-parsimony tree based on 26S rDNA D1/D2 domain sequences is available as supplementary material with the online version of this paper.






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