HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Figures and Table Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gupta, S. K. Articles by Lal, R. Search for Related Content PubMed PubMed Citation Articles by Gupta, S. K. Articles by Lal, R. Agricola Articles by Gupta, S. K. Articles by Lal, R.

Novosphingobium panipatense sp. nov. and Novosphingobium mathurense sp. nov., from oil-contaminated soil

Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi-110007, India

Correspondence
Rup Lal
duzdel{at}vsnl.com

In an ongoing attempt to analyse the diversity of culturable micro-organisms from oil-contaminated soil, two yellow-pigmented, Gram-negative, halophilic bacterial strains (SM16^T and SM117^T) were isolated. These two strains were characterized using a polyphasic approach. Strains SM16^T and SM117^T showed a range of phenotypic and chemotaxonomic properties that were consistent with those of members of the genus Novosphingobium. Both strains contained sphingoglycolipids, thus confirming that they belong to the Alphaproteobacteria. Furthermore, the polar lipid profile consisted of phosphatidylglycerol, phosphatidyldimethylethanolamine and phosphatidylethanolamine, with minor amounts of phosphatidyldimethylethanolamine, phosphatidylcholine and phosphatidylmonomethylethanolamine. Spermidine was the major polyamine in the cell wall, a characteristic feature of members of the genus Novosphingobium. Fatty acid analysis revealed the presence of C_{18 : 1}7c, C_{16 : 0} and C_{14 : 0} 2-OH in both isolates, a characteristic feature of sphingomonads. 16S rRNA gene sequence similarities with the type strains of the most closely related species of the genus Novosphingobium (Novosphingobium pentaromativorans and Novosphingobium resinovorum) were less than 98.8 % for both strains. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains SM16^T and SM117^T fall in the clade represented by the genus Novosphingobium. Strains SM16^T and SM117^T shared 98.8 % similarity in their 16S rRNA gene sequences and their mean level of DNA–DNA relatedness was 8.5 %. Strains SM16^T and SM117^T differed from each other with respect to their morphological, physiological and chemotaxonomic properties. Thus, these results indicate that strains SM16^T and SM117^T belong to separate species of the genus Novosphingobium, for which the names Novosphingobium panipatense sp. nov. and Novosphingobium mathurense sp. nov. are proposed; the type strains are SM16^T (=CCM 7472^T =MTCC9019^T) and SM117^T (=CCM 7473^T =MTCC9020^T), respectively.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strain SM16^T and SM117^T are respectively EF424402 and EF424403.

Transmission electron micrographs of negatively stained cells, TLC polar lipid profiles of strains SM16^T and SM117^T and DNA–DNA relatedness values between strains SM16^T and SM117^T and related strains are available as supplementary material with the online version of this paper.

This article has been cited by other articles:

				H. Kumari, S. K. Gupta, S. Jindal, P. Katoch, and R. Lal Sphingobium lactosutens sp. nov., isolated from a hexachlorocyclohexane dump site and Sphingobium abikonense sp. nov., isolated from oil-contaminated soil Int J Syst Evol Microbiol, September 1, 2009; 59(9): 2291 - 2296. [Abstract] [Full Text] [PDF]