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Paenibacillus siamensis sp. nov., Paenibacillus septentrionalis sp. nov. and Paenibacillus montaniterrae sp. nov., xylanase-producing bacteria from Thai soils

¹ Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
² Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand
³ Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yusong, Daejeon 305-806, Republic of Korea

Correspondence
Ancharida Akaracharanya
sanchari{at}chula.ac.th

Three strains of xylanase-producing bacteria, S5-3^T, X13-1^T and MXC2-2^T, isolated from soils in Thailand, were characterized taxonomically based on their phenotypic and chemotaxonomic characteristics and 16S rRNA gene sequence comparisons. They were Gram-positive, facultatively anaerobic, spore-forming, rod-shaped bacteria. They contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The DNA G+C contents were respectively 45.8, 47.3 and 48.8 mol%. The predominant isoprenoid quinone was MK-7, and anteiso-C_{15 : 0} and C_{16 : 0} were the dominant cellular fatty acids. Phylogenetic analyses based on 16S rRNA gene sequence comparison showed that they were affiliated to the genus Paenibacillus. Strains S5-3^T, X13-1^T and MXC2-2^T were closely related to Paenibacillus granivorans A30^T and Paenibacillus agaridevorans DSM 1355^T (94.7–98.6 % 16S rRNA gene sequence similarity). The similarity between the three novel strains ranged from 96.3 to 98.4 %. DNA–DNA relatedness, DNA G+C contents and some phenotypic characteristics could clearly distinguish the strains from each other and from related Paenibacillus species. Therefore, strains S5-3^T, X13-1^T and MXC2-2^T represent novel species of the genus Paenibacillus, for which the names Paenibacillus siamensis sp. nov. (type strain S5-3^T =KCTC 13038^T =PCU 279^T =TISTR 1831^T), Paenibacillus septentrionalis sp. nov. (type strain X13-1^T =KCTC 13039^T =PCU 280^T =TISTR 1830^T) and Paenibacillus montaniterrae sp. nov. (type strain MXC2-2^T =KCTC 13036^T =PCU 281^T =TISTR 1836^T) are proposed.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains S5-3^T, MXC2-2^T and X13-1^T are AB295645–AB295647, respectively.

Extended 16S rRNA gene sequence-based neighbour-joining and maximum-parsimony trees are available as supplementary material with the online version of this paper.

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