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Paenibacillus contaminans sp. nov., isolated from a contaminated laboratory plate

¹ Department of Soil Environmental Science, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan
² Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan
³ Department of Aquaculture, National Kaohsiung Marine University, Kaohsiung, Taiwan

Correspondence
Wen-Ming Chen
p62365{at}ms28.hinet.net

A bacterial strain, designated CKOBP-6^T, isolated from a contaminated laboratory plate, was characterized by using a polyphasic taxonomic approach. Chemotaxonomic and morphological properties of the strain were consistent with its classification in the genus Paenibacillus. Cells were Gram-variable, aerobic, sporulating, motile rods. 16S rRNA gene sequence analysis demonstrated that this novel isolate was unique, showing 94.6 % sequence similarity to Paenibacillus chitinolyticus NBRC 15660^T and lower levels of similarity to Paenibacillus gansuensis KCTC 3950^T (93.8 %) and Paenibacillus terrigena A35^T (93.8 %). The novel isolate could be distinguished from the type strains of all of these species based on a range of phenotypic data. The major cellular phospholipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and one unknown phosphoglycolipid. The predominant isoprenologue was an unsaturated menaquinone with seven isoprene units (MK-7). The major fatty acids of strain CKOBP-6^T were C_{16 : 0} (12.0 %), anteiso-C_{15 : 0} (52.0 %) and iso-C_{16 : 0} (16.4 %). The G+C content of the genomic DNA was 51.2 mol%. It is evident from the genotypic and phenotypic data that strain CKOBP-6^T should be classified as a representative of a novel species of the genus Paenibacillus, for which the name Paenibacillus contaminans sp. nov. is proposed. The type strain is CKOBP-6^T (=BCRC 17728^T=LMG 24216^T).

An extended neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showing the relationship between strain CKOBP-6^T and species of the genus Paenibacillus is available as supplementary material with the online version of this paper.