HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Figures and Tables Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hahn, M. W. Search for Related Content PubMed PubMed Citation Articles by Hahn, M. W. Agricola Articles by Hahn, M. W.

Description of seven candidate species affiliated with the phylum Actinobacteria, representing planktonic freshwater bacteria

Institute for Limnology, Austrian Academy of Sciences, Mondseestrasse 9, A-5310 Mondsee, Austria

Correspondence
Martin W. Hahn
martin.hahn{at}oeaw.ac.at

Actinobacteria comprise a substantial fraction of the bacterioplankton in freshwater lakes and streams. Numerous cultivation-independent investigations have retrieved actinobacterial 16S rRNA gene sequences from such habitats. The taxa detected in freshwater habitats are usually absent from terrestrial and marine systems. So far, none of the indigenous freshwater lineages is represented by a taxon with a validly published name. The seven organisms for which Candidatus status is described here were isolated from freshwater lakes and ponds located in tropical, subtropical and temperate climatic zones. Phylogenetic analyses demonstrated that they are affiliated with one of the actinobacterial lineages indigenous to freshwater bacterioplankton. The seven novel taxa could only be cultivated to date as mixed cultures that also contain non-actinobacterial strains. Due to the lack of pure cultures, I propose to establish the candidate species ‘Candidatus Planktoluna difficilis’, ‘Candidatus Aquiluna rubra’, ‘Candidatus Flaviluna lacus’, ‘Candidatus Rhodoluna limnophila’, ‘Candidatus Rhodoluna planktonica’, ‘Candidatus Rhodoluna lacicola’ and ‘Candidatus Limnoluna rubra’ for these taxa.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains representing ‘Candidatus Rhodoluna planktonica’, ‘Candidatus Aquiluna rubra’, ‘Candidatus Rhodoluna limnophila’, ‘Candidatus Rhodoluna lacicola’, ‘Candidatus Planktoluna difficilis’, ‘Candidatus Flaviluna lacus’ and ‘Candidatus Limnoluna rubra’ are AJ565415–AJ565417, AM182889, AM939566, AM939567 and AM943659, respectively.

A photo of growth of one of the novel strains, a 16S rRNA gene sequence-based neighbour-joining tree, further details of strain isolation sites and a 16S rRNA gene sequence similarity matrix are available as supplementary material with the online version of this paper.

This article has been cited by other articles:

				J. Jezbera, A. K. Sharma, U. Brandt, W. F. Doolittle, and M. W. Hahn 'Candidatus Planktophila limnetica', an actinobacterium representing one of the most numerically important taxa in freshwater bacterioplankton Int J Syst Evol Microbiol, November 1, 2009; 59(11): 2864 - 2869. [Abstract] [Full Text] [PDF]