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Int J Syst Evol Microbiol 58 (2008), 2180-2185; DOI  10.1099/ijs.0.65778-0
© 2008 International Union of Microbiological Societies

Acetobacter fabarum sp. nov., an acetic acid bacterium from a Ghanaian cocoa bean heap fermentation

Ilse Cleenwerck1, Ángel Gonzalez2, Nicholas Camu2, Katrien Engelbeen1, Paul De Vos1,3 and Luc De Vuyst2

1 BCCM/LMG Bacteria Collection, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium
2 Research Group of Industrial Microbiology and Food Biotechnology, Department of Applied Biological Sciences and Engineering, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium
3 Laboratory of Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium

Correspondence
Ilse Cleenwerck
Ilse.Cleenwerck{at}ugent.be

Six acetic acid bacterial isolates, obtained during a study of the microbial diversity of spontaneous fermentations of Ghanaian cocoa beans, were subjected to a polyphasic taxonomic study. (GTG)5-PCR fingerprinting grouped the isolates together, but they could not be identified using this method. Phylogenetic analysis based on 16S rRNA gene sequences allocated the isolates to the genus Acetobacter and revealed Acetobacter lovaniensis, Acetobacter ghanensis and Acetobacter syzygii to be nearest neighbours. DNA–DNA hybridizations demonstrated that the isolates belonged to a single novel genospecies that could be differentiated from its phylogenetically nearest neighbours by the following phenotypic characteristics: no production of 2-keto-D-gluconic acid from D-glucose; growth on methanol and D-xylose, but not on maltose, as sole carbon sources; no growth on yeast extract with 30 % D-glucose; and weak growth at 37 °C. The DNA G+C contents of four selected strains were 56.8–58.0 mol%. The results obtained prove that the isolates should be classified as representatives of a novel Acetobacter species, for which the name Acetobacter fabarum sp. nov. is proposed. The type strain is strain 985T (=R-36330T =LMG 24244T =DSM 19596T).

Abbreviations: AAB, acetic acid bacteria

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains 985T and 1145 are AM905849 and AM905850, respectively.

A maximum-parsimony tree based on nearly complete 16S rRNA gene sequences and additional differential characteristics of A. fabarum and phylogenetically related species are available as supplementary material with the online version of this paper.






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