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Int J Syst Evol Microbiol 58 (2008), 2158-2163; DOI  10.1099/ijs.0.65632-0
© 2008 International Union of Microbiological Societies

Rhizobium oryzae sp. nov., isolated from the wild rice Oryza alta

Guixiang Peng1, Qinghua Yuan2, Huaxing Li1, Wu Zhang2 and Zhiyuan Tan2

1 College of Resources and Environment, South China Agricultural University, Guangzhou 510642, PR China
2 Provincial Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, PR China

Correspondence
Zhiyuan Tan
zytan{at}scau.edu.cn

During a study of endophytic nitrogen-fixing bacteria present in the wild rice species Oryza alta, eight novel isolates were obtained from surface-sterilized roots and classified in the genus Rhizobium on the basis of almost-complete 16S rRNA gene sequence analysis. These strains can nodulate Phaseolus vulgaris and Glycine max. The highly similar protein patterns, DNA fingerprint patterns of insertion sequence-based PCR (IS-PCR) and DNA–DNA hybridizations showed that these novel isolates were members of the same species. The closest phylogenetic relatives of the representative strain Alt 505T of the novel group were Rhizobium etli CFN 42T and Rhizobium indigoferae CCBAU 71714T, with 96.2 and 96.0 % 16S rRNA gene sequence similarity, respectively. Low DNA–DNA relatedness with the type strains of R. etli, R. indigoferae, Rhizobium hainanense, Rhizobium mongolense and Rhizobium galegae and differences in IS-PCR fingerprinting patterns, SDS-PAGE of proteins, antibiotic resistance, phenotypic tests and comparison of cellular fatty acids with Rhizobium species indicated that the novel group of isolates were distinct from previously described species. Based on these results, we propose to place them in a novel species, as Rhizobium oryzae sp. nov. The type strain is Alt 505T (=LMG 24253T =CGMCC 1.7048T).

Abbreviations: IS-PCR, insertion sequence-based PCR

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains Alt 505T and Alt 501 are respectively EU056823 and EU056822.

A transmission electron micrograph of cells of strain Alt 505T, detailed DNA–DNA hybridization results and a list of characters of two representative novel isolates are available as supplementary material with the online version of this paper.






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