HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Figures Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yachai, M. Articles by Valyasevi, R. Search for Related Content PubMed PubMed Citation Articles by Yachai, M. Articles by Valyasevi, R. Agricola Articles by Yachai, M. Articles by Valyasevi, R.

Halobacterium piscisalsi sp. nov., from fermented fish (pla-ra) in Thailand

¹ Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand
² Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand
³ Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
⁴ National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Klong 1, Klong Luang, Pathumthani 12120, Thailand

Correspondence
Wonnop Visessanguan
wonnop{at}biotec.or.th

A Gram-negative, motile, rod-shaped, extremely halophilic archaeon, designated strain HPC1-2^T, was isolated from pla-ra, a salt-fermented fish product of Thailand. Strain HPC1-2^T was able to grow at 20–60 °C (optimum at 37–40 °C), at 2.6–5.1 M NaCl (optimum at 3.4–4.3 M NaCl) and at pH 5.0–8.0 (optimum at pH 7.0–7.5). Hypotonic treatment with less than 1.7 M NaCl caused cell lysis. The major polar lipids of the isolate were C₂₀C₂₀ derivatives of phosphatidylglycerol, methylated phosphatidylglycerol phosphate, phosphatidylglycerol sulfate, triglycosyl diether, sulfated triglycosyl diether and sulfated tetraglycosyl diether. The G+C content of the DNA was 65.5 mol%. 16S rRNA gene sequence analysis indicated that the isolate represented a member of the genus Halobacterium in the family Halobacteriaceae. Based on 16S rRNA gene sequence similarity, strain HPC1-2^T was related most closely to Halobacterium salinarum DSM 3754^T (99.2 %) and Halobacterium jilantaiense JCM 13558^T (97.8 %). However, low levels of DNA–DNA relatedness suggested that strain HPC1-2^T was genotypically different from these closely related type strains. Strain HPC1-2^T could also be differentiated based on physiological and biochemical characteristics. Therefore, strain HPC1-2^T is considered to represent a novel species of the genus Halobacterium, for which the name Halobacterium piscisalsi sp. nov. is proposed. The type strain is HPC1-2^T (=BCC 24372^T=JCM 14661^T=PCU 302^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain HPC1-2^T is AB285020.

Maximum-parsimony and maximum-likelihood phylogenetic trees showing the relationships between strain HPC1-2^T and related archaeal species based on 16S rRNA gene sequences are available with the online version of this paper.

This article has been cited by other articles:

				A. Oren, D. R. Arahal, and A. Ventosa Emended descriptions of genera of the family Halobacteriaceae Int J Syst Evol Microbiol, March 1, 2009; 59(3): 637 - 642. [Abstract] [Full Text] [PDF]