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Clostridium amylolyticum sp. nov., isolated from H₂-producing UASB granules

¹ State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China
² Graduate School of Chinese Academy of Sciences, Beijing 100101, PR China

Correspondence
Xiuzhu Dong
dongxz{at}sun.im.ac.cn

A Gram-stain-positive, strictly anaerobic, mesophilic, amylolytic, rod-shaped bacterium, designated strain SW408^T, was isolated from a laboratory-scale H₂-producing upflow anaerobic sludge blanket reactor. The strain grew at 24–45 °C (no growth at or below 22 °C or at or above 47 °C), with optimum growth at 37 °C. The pH range for growth was 4.0–9.0 (no growth at or below pH 3.6 or at or above pH 9.3), with optimum growth at pH 7.0. Starch, cellobiose, glucose, fructose, galactose, lactose, maltose, mannose, ribose and sucrose supported growth. The major end products from glucose fermentation were ethanol, acetate, hydrogen and carbon dioxide. Abundant H₂ was produced from starch fermentation. The DNA G+C content was 33.1 mol% (T_m method). Phylogenetic analysis based on 16S rRNA gene sequence analysis showed that the bacterium represents a previously unrecognized species within Clostridium rRNA cluster I and is most closely related to the type strain of Clostridium frigidicarnis (94.9 % similarity). On the basis of phenotypic, genotypic and phylogenetic characteristics, strain SW408^T was identified as a representative of a novel species of the genus Clostridium, for which the name Clostridium amylolyticum sp. nov. is proposed. The type strain is SW408^T (=JCM 14823^T=AS 1.5069^T=CGMCC 1.5069^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Clostridium amylolyticum SW408^T is EU037903.

An electron micrograph of a negatively stained cell of strain SW408^T (Fig. S1) and a table showing the cellular fatty acid composition of strain SW408^T and phylogenetically related clostridia (Table S1) are available with the online version of this paper.