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1 School of Earth and Environmental Sciences, Seoul National University, Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea
2 Marine Environmental Research Department, Korea Ocean Research and Development Institute, Ansan 426-744, Republic of Korea
Correspondence
Byung Cheol Cho
bccho{at}snu.ac.kr
A rod-shaped marine bacterium, designated CL-Z59T, was isolated from seawater enriched with zooplankton. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CL-Z59T belongs to the genus Nocardioides, the highest level of sequence similarity (99.2 %) being found with respect to Nocardioides marinisabuli KCCM 42681T. However, DNA–DNA hybridization experiments between strain CL-Z59T and N. marinisabuli KCCM 42681T revealed a mean relatedness value of 33 %. Cells were Gram-positive, strictly aerobic and non-motile. Strain CL-Z59T grew optimally at pH 6–7 and 25–30 °C and at a NaCl concentration of 3 %. The isolate was characterized chemotaxonomically as having LL-diaminopimelic acid in the cell wall. The major isoprenoid quinone was MK-8(H4) and the predominant cellular fatty acid was iso-C16 : 0. The DNA G+C content was 73.3 mol%. On the basis of phylogenetic, genotypic and phenotypic data, strain CL-Z59T represents a novel species of the genus Nocardioides, for which the name Nocardioides salarius sp. nov. is proposed. The type strain is CL-Z59T (=KCCM 42320T=DSM 18239T).
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