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Cloacibacillus evryensis gen. nov., sp. nov., a novel asaccharolytic, mesophilic, amino-acid-degrading bacterium within the phylum ‘Synergistetes’, isolated from an anaerobic sludge digester

¹ CEA-Genoscope, 2 Rue Gaston Crémieux, 91057 Evry, France
² CNRS-UMR 8030, 2 Rue Gaston Crémieux, 91057 Evry, France
³ Laboratoire de Neurogénétique Moléculaire, INSERM, U798, Evry 91057, France
⁴ Université Paris XI, France
⁵ Genopole de l'Institut Pasteur, Paris, France
⁶ Cemagref, Hydrosystèmes et Bioprocédés, Parc de Tourvoie, BP 44, 92163 Antony Cedex, France
⁷ Université d'Evry Val d'Essonne, 2 Rue Gaston Crémieux, 91057 Evry, France

Correspondence
Abdelghani Sghir
sghir{at}genoscope.cns.fr

A novel anaerobic, mesophilic, amino-acid-utilizing bacterium, strain 158^T, was isolated from an anaerobic digester of a wastewater treatment plant. Cells of strain 158^T were non-motile, rod-shaped (2.0–3.0 x 0.8–1.0 µm) and stained Gram-negative. Optimal growth occurred at 37 °C and pH 7.0 in an anaerobic basal medium containing 1 % Casamino acids. Strain 158^T fermented arginine, histidine, lysine and serine and showed growth on yeast extract, brain-heart infusion (BHI) medium and tryptone, but not on carbohydrates, organic acids or alcohols. The end products of degradation were: acetate, butyrate, H₂ and CO₂ from arginine; acetate, propionate, butyrate, H₂ and CO₂ from lysine; and acetate, propionate, butyrate, valerate, H₂ and CO₂ from histidine, serine, BHI medium, Casamino acids and tryptone. The DNA G+C content was 55.8 mol%. The 16S rRNA gene sequence of strain 158^T showed only 92.6 % sequence similarity with that of Synergistes jonesii, the only described species of the ‘Synergistes’ group. The major cellular fatty acids were iso-C_{15 : 0} (16.63 %), iso-C_{15 : 0} 3-OH (12.41 %) and C_{17 : 1}6c (9.46 %) and the polar fatty acids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylmonomethylamine; these fatty acid profiles did not resemble those of any recognized bacterial species. Due to the considerable differences in genotypic, phenotypic and phylogenetic characteristics between strain 158^T and those of its nearest relative, it is proposed that strain 158^T represents a novel species in a new genus, Cloacibacillus evryensis gen. nov., sp. nov., in the phylum ‘Synergistetes’. The type strain is 158^T (=DSM 19522^T=JCM 14828^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Cloacibacillus evryensis strain 158^T is CU463952.