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Aquisalibacillus elongatus gen. nov., sp. nov., a moderately halophilic bacterium of the family Bacillaceae isolated from a saline lake

¹ Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain
² State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100080 Beijing, PR China
³ Institute for Microbial Biotechnology and Metagenomics, University of the Western Cape, Bellville 7535, Cape Town, South Africa
⁴ Genencor International BV, 2333 CN Leiden, The Netherlands
⁵ Department of Infection, Immunity and Inflammation, University of Leicester, Leicester LE1 9HN, UK

Correspondence
M. C. Márquez
cmarquez{at}us.es

A novel moderately halophilic, non-motile, rod-shaped bacterium was isolated from a saline lake, Lake Shangmatala, in the Inner Mongolia Autonomous Region, China. This bacterium, designated SH4s^T, was strictly aerobic, catalase-positive and oxidase-negative. It grew at salinities of 3–20 % (w/v) NaCl, with an optimum at 10 % (w/v) NaCl. The cell-wall peptidoglycan was of the A4β type, based on L-Orn–D-Asp, and the major quinone was a menaquinone with seven isoprene units (MK-7). The major fatty acids were iso-C_{16 : 0} and iso-C_{15 : 0}. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, a glycolipid and four different unidentified phospholipids. The DNA G+C content was 45.9 mol%. In a maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences, strain SH4s^T was found to belong to the family Bacillaceae and to be most closely related to members of the genera Filobacillus (95.9 % sequence similarity), Piscibacillus (95.7 %) and Tenuibacillus (95.4 %). DNA–DNA hybridization experiments revealed 10 % relatedness (12 %, reciprocally) between strain SH4s^T and Filobacillus milosensis DSM 13259^T, the sole species of the genus. All of these data show that strain SH4s^T represents a novel genus and species in the family Bacillaceae, for which the name Aquisalibacillus elongatus gen. nov., sp. nov. is proposed. The type strain of Aquisalibacillus elongatus is SH4s^T (=CCM 7366^T =CECT 7149^T =DSM 18090^T).

Neighbour-joining and maximum-likelihood 16S rRNA gene sequence-based phylogenetic trees and a two-dimensional thin-layer chromatogram of the polar lipids of SH4s^T are available as supplementary material with the online version of this paper.

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