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Int J Syst Evol Microbiol 58 (2008), 1911-1915; DOI  10.1099/ijs.0.65459-0
© 2008 International Union of Microbiological Societies

Pseudomonas xiamenensis sp. nov., a denitrifying bacterium isolated from activated sludge

Qiliang Lai and Zongze Shao

Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, People's Republic of China

Correspondence
Zongze Shao
shaozz{at}163.com

A taxonomic study was carried out on strain C10-2T, a moderately halophilic denitrifier isolated from activated sludge samples collected in China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain C10-2T belonged to the genus Pseudomonas, with highest sequence similarity of 98.1 and 97.7 % to ‘Pseudomonas denitrificans’ IAM 12023 and Pseudomonas pertucinogena JCM 11590T, respectively. The gyrB, rpoD and rpoB gene sequence similarity between strain C10-2T and P. pertucinogena JCM 11590T was respectively 84.1, 79.0 and 88.2 %. Phylogenetic trees based on these housekeeping genes showed that strain C10-2T and P. pertucinogena JCM 11590T form a clade at the periphery of the genus Pseudomonas. The DNA–DNA hybridization value between strain C10-2T and P. pertucinogena JCM 11590T was 30–32 %. The G+C content of the chromosomal DNA was 61.2 mol%. The combined genotypic and phenotypic data show that strain C10-2T represents a novel species of the genus Pseudomonas, for which the name Pseudomonas xiamenensis sp. nov. is proposed, with the type strain C10-2T (=CGMCC 1.6446 =JCM 13530T =MCCC 1A00089T).

The GenBank/EMBL/DDBJ accession numbers for the nucleotide sequences reported in this study are DQ088664 (strain C10-2T 16S rRNA gene), DQ350612 (strain C10-2T partial gyrB gene), EF596884 (strain C10-2T partial rpoD gene), EF667149 (strain C10-2T partial rpoB gene), EF673695 (P. pertucinogena JCM 11590T 16S rRNA gene), DQ350613 (P. pertucinogena JCM 11950T partial gyrB gene) and EF596883 (P. pertucinogena JCM 11950T partial rpoD gene).

Transmission electron micrographs of cells of strain C10-2T and dendrograms showing the phylogenetic position of the strain plus related species based on 16S rRNA, gyrB, rpoD and rpoB gene sequences, detailed cellular fatty acid profiles and DNA–DNA hybridization results are available as supplementary material with the online version of this paper.






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