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Int J Syst Evol Microbiol 58 (2008), 1864-1868; DOI  10.1099/ijs.0.65810-0
© 2008 International Union of Microbiological Societies

Nocardioides fonticola sp. nov., a novel actinomycete isolated from spring water

Jui-Hsing Chou1, Nian-Tsz Cho2, A. B. Arun1, Chiu-Chung Young1 and Wen-Ming Chen2

1 Department of Soil Environmental Science, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan, ROC
2 Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, 142 Hai-Chuan Road, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC

Correspondence
Wen-Ming Chen
p62365{at}ms28.hinet.net

A Gram-positive, rod-shaped, yellow-pigmented actinomycete, designated strain NAA-13T, was isolated from a freshwater spring located in Kaoshiung, southern Taiwan. This strain was characterized using a polyphasic taxonomic approach. The organism had chemotaxonomic and morphological properties consistent with its classification in the genus Nocardioides. The strain contained LL-diaminopimelic acid as the principal diamino acid of the peptidoglycan, MK-8(H4) as the major menaquinone, and iso-C16 : 0 and C17 : 0 as the predominant fatty acids. 16S rRNA gene sequence studies showed that the strain formed a monophyletic branch at the periphery of the evolutionary radiation occupied by the genus Nocardioides; its closest neighbours were Nocardioides insulae DS-51T (94.9 % similarity), Nocardioides pyridinolyticus KCTC 0074BPT (96.5 % similarity) and Nocardioides aquiterrae GW-9T (96.6 % similarity). The DNA G+C content of strain NAA-13T was 71.8 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain NAA-13T represents a novel species of the genus Nocardioides, for which the name Nocardioides fonticola sp. nov. is proposed. The type strain is NAA-13T (=LMG 24213T=BCRC 16874T).


Abbreviations: DAP, diaminopimelic acid

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain NAA-13T is EF626689.

A table showing the cellular fatty acid composition of strain NAA-13T grown on R2A agar is available with the online version of this paper.




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J.-H. Yoon, S.-J. Kang, S. Park, W. Kim, and T.-K. Oh
Nocardioides caeni sp. nov., isolated from wastewater
Int J Syst Evol Microbiol, November 1, 2009; 59(11): 2794 - 2797.
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