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Thermobifida halotolerans sp. nov., isolated from a salt mine sample, and emended description of the genus Thermobifida

Ling-Ling Yang^1,, Shu-Kun Tang^1,, Yu-Qin Zhang^1,2, Xiao-Yang Zhi¹, Dong Wang³, Li-Hua Xu¹ and Wen-Jun Li^1,4

¹ The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China
² Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China
³ Science College, Honghe University, Mengzi, Yunnan 661100, PR China
⁴ Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China

Correspondence
Wen-Jun Li
wjli{at}ynu.edu.cn
or
liact{at}hotmail.com

The actinomycete strain YIM 90462^T was isolated from a salt mine sample collected from Yunnan province, south-west China. Comparative 16S rRNA gene sequence analysis demonstrated that the isolate, which had sequence similarities of more than 97 % to Thermobifida alba, Thermobifida fusca and Thermobifida cellulosilytica, was a candidate member of the genus Thermobifida. Subsequently, a polyphasic approach was used to clarify the taxonomic position of strain YIM 90462^T. Its diagnostic diamino acid was meso-diaminopimelic acid and whole-cell sugars contained galactose, xylose and glucose. The predominant menaquinones were MK-10(H₆) and MK-10(H₈). The major fatty acids were iso-C_{16 : 0} and anteiso-C_{17 : 0}. Results of DNA–DNA hybridizations and physiological and biochemical tests enabled genotypic and phenotypic differentiation of strain YIM 90462^T from the three Thermobifida species with validly published names. Consequently, it is proposed that strain YIM 90462^T should be recognized as a representative of a novel species of Thermobifida, Thermobifida halotolerans sp. nov. The type strain is YIM 90462^T (=KCTC 19123^T =DSM 44931^T). In addition, an emended description of the genus Thermobifida is presented.

These authors contributed equally to this work.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain YIM 90462^T is EU250489.

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