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Spongiispira norvegica gen. nov., sp. nov., a marine bacterium isolated from the boreal sponge Isops phlegraei

¹ Berlin University of Technology, Environmental Microbiology Group, Franklinstrasse 29, Sekr. FR 1-2, D-10587 Berlin, Germany
² Hamburg University of Technology, Technical Microbiology, Kasernenstrasse 12, D-21073 Hamburg, Germany
³ University of Hamburg, Institute of Biogeochemistry and Marine Chemistry, Bundesstrasse 55, D-20146 Hamburg, Germany
⁴ Research Center Ocean Margins, University of Bremen, Klagenfurter Strasse, D-28359 Bremen, Germany
⁵ Anagnostec GmbH, Am Mühlenberg 11, D-14476 Potsdam-Golm, Germany
⁶ Berlin University of Technology, Biochemistry and Molecular Biology Group, Franklinstrasse 29, Sekr. OE2, D-10587 Berlin, Germany
⁷ Novozymes A/S, Bacterial Screening, Krogshøjvej 36, DK-2880 Bagsværd, Denmark
⁸ Max-Planck Institute for Molecular Genetics, Ihnestrasse 63-73, D-14195 Berlin, Germany

Correspondence
Ines Kaesler
ineskaesler{at}web.de

The bacterial strain Gp_4_7.1^T, isolated from the marine sponge Isops phlegraei collected at the Sula Ridge off the Norwegian coast, was characterized. The isolate was a motile spirillum that was monopolarly and monotrichously flagellated. It was aerobic, Gram-negative, oxidase-positive and catalase-negative. Optimal growth occurred between 20 and 30 °C, at pH 7–8 and with a salt concentration of 2–3 % (w/v). The isolate showed a relatively restricted nutritional profile. Substrate utilization tests were only positive for arabinose. Enzyme tests were positive for esterase lipase C8, lipase C14, leucine arylamidase and naphthol-AS-BI-phosphohydrolase. The strain was not able to reduce nitrate. The major cellular fatty acids were C_{16 : 1}7 and C_{16 : 0}. The DNA G+C content was 62.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparison classified the strain as a member of the order Oceanospirillales in the class Gammaproteobacteria. Strain Gp_4_7.1^T formed a distinct phyletic line with less than 94 % 16S rRNA gene sequence similarity to its closest relatives with validly published names. Based on the determined data, it is proposed that the strain represents a novel species in a new genus, Spongiispira norvegica gen. nov., sp. nov.; the type strain of Spongiispira norvegica is Gp_4_7.1^T (=DSM 17749^T =NCIMB 14401^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Spongiispira norvegica strain Gp_4_7.1^T is AM117931.