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Actinomadura bangladeshensis sp. nov. and Actinomadura chokoriensis sp. nov.

Ismet Ara¹, Atsuko Matsumoto², Mohammad Abdul Bakir³, Takuji Kudo³, Satoshi mura^1,2 and Yoko Takahashi¹

¹ Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan
² The Kitasato Institute, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8642, Japan
³ Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan

Correspondence
Yoko Takahashi
ytakaha{at}lisci.kitasato-u.ac.jp

The taxonomic position of two soil isolates, 3-46-b(3)^T and 3-45-a(11)^T, was clarified based on data from a polyphasic study. The organisms showed a combination of chemotaxonomic and morphological properties typical of the genus Actinomadura. They formed distinct phyletic lines in the Actinomadura 16S rRNA gene tree and were closely associated with the type strains of Actinomadura meyerae (sequence similarity of 98.3–98.5 %), Actinomadura napierensis (98.1–98.3 %) and Actinomadura latina (96.4–96.8 %). The level of 16S rRNA gene sequence similarity between the new isolates was 99.1 %. The level of DNA–DNA hybridization between strains 3-46-b(3)^T and 3-45-a(11)^T was 43.6 % and levels of relatedness between the two new isolates and the type strains of A. meyerae and A. napierensis were 21.0–27.3 %. On the basis of phenotypic and genotypic properties, the new isolates could be differentiated from each other and from their closest phylogenetic relatives. It is proposed that the organisms be classified as representing two novel species of the genus Actinomadura. The names proposed for these taxa are Actinomadura bangladeshensis sp. nov. [type strain 3-46-b(3)^T =JCM 13933^T =MTCC8057^T] and Actinomadura chokoriensis sp. nov. [type strain 3-45-a(11)^T =JCM 13932^T =MTCC8056^T].

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains 3-46-b(3)^T and 3-45-a(11)^T are AB331652 and AB331730, respectively.

A neighbour-joining tree based on nearly-complete 16S rRNA gene sequences showing the relationships between strains 3-46-b(3)^T, 3-45-a(11)^T and the type strains of all recognized species of the genus Actinomadura is available as supplementary material with the online version of this paper.