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Int J Syst Evol Microbiol 58 (2008), 1625-1629; DOI  10.1099/ijs.0.65677-0
© 2008 International Union of Microbiological Societies

Lactobacillus senmaizukei sp. nov., isolated from Japanese pickle

Kazumi Hiraga1, Yoshie Ueno2, Sirapan Sukontasing3, Somboon Tanasupawat3 and Kohei Oda1

1 Department of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Goshokaido-cho, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan
2 Kyoto Prefectural Technology Center for Small and Medium Enterprise, 134 Chudoji, Minamimachi, Shimogyo-ku, Kyoto 600-8813, Japan
3 Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand

Correspondence
Kohei Oda
bika{at}kit.ac.jp

The taxonomic position of a lactic acid bacterium, strain L13T, isolated from senmaizuke, a Japanese traditional pickle, was studied. This strain was a heterofermentative, facultatively anaerobic, Gram-positive, non-spore-forming, non-motile, rod-shaped bacterium. It produced DL-lactic acid from glucose. Phylogenetic analysis of its 16S rRNA gene sequence and physiological and biochemical characteristics indicated that the strain was a member of the genus Lactobacillus. Based on 16S rRNA gene sequence similarity, strain L13T was closely related to Lactobacillus hammesii CIP 108387T (98.9 %) and Lactobacillus parabrevis ATCC 53295T (98.5 %). In addition, the rpoA gene sequence of strain L13T was closely related to those of L. hammesii CIP 108387T (92.2 %) and L. parabrevis ATCC 53295T (92.0 %). However, DNA–DNA hybridization of strain L13T with these two strains revealed that strain L13T represents a separate genomic species. The DNA G+C content of strain L13T was 46 mol% and the peptidoglycan was of the A4{alpha} L-Lys–D-Asp type. Thus, these data indicate that strain L13T represents a novel species of the genus Lactobacillus, for which the name Lactobacillus senmaizukei is proposed. The type strain is L13T (=NBRC 103853T=TISTR 1847T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and rpoA gene sequences of strain L13T are AB297927 and AB359074, respectively. The accession number for the rpoA gene sequence of Lactobacillus parabrevis ATCC 53295T is AB359076.

Phylogenetic trees based on 16S rRNA gene sequences derived by the neighbour-joining (Supplementary Fig. S1), maximum-likelihood (Supplementary Fig. S2) and maximum-parsimony (Supplementary Fig. S3) methods showing the position of Lactobacillus senmaizukei L13T among selected lactobacilli are available with the online version of this paper.






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