HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Figure Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Google Scholar Articles by Smith, J. L. Articles by Cary, S. C. PubMed PubMed Citation Articles by Smith, J. L. Articles by Cary, S. C. Agricola Articles by Smith, J. L. Articles by Cary, S. C.

Nautilia profundicola sp. nov., a thermophilic, sulfur-reducing epsilonproteobacterium from deep-sea hydrothermal vents

¹ University of Delaware, College of Marine Studies, Lewes, DE 19958, USA
² Delaware Biotechnology Institute, Newark, DE, USA
³ Savannah River Ecology Laboratory, University of Georgia, Aiken, SC, USA
⁴ University of Waikato, Hamilton, New Zealand

Correspondence
S. Craig Cary
caryc{at}udel.edu

A thermophilic, strictly anaerobic, sulfur-reducing epsilonproteobacterium (strain AmH^T) isolated from deep-sea hydrothermal vents is described. Cells were motile, Gram-negative rods. Growth was observed at 30–55 °C, pH 6.0–9.0 and 2–5 % (w/v) NaCl. Chemolithoautotrophic growth occurred with molecular hydrogen or formate as the electron donor and elemental sulfur as the electron acceptor, producing hydrogen sulfide. Heterotrophic and mixotrophic growth occurred with formate as a source of carbon. The dominant phospholipid fatty acids were C_{18 : 1}7c (73.26 % of the total), C_{16 : 1}7c (12.70 %) and C_{16 : 0} (12.27 %). The genomic DNA G+C content was 33.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences placed strain AmH^T within the family Nautiliaceae of the Epsilonproteobacteria. DNA–DNA hybridization experiments between strain AmH^T and Nautilia lithotrophica DSM 13520^T revealed a level of relatedness of 34.6 % between the two strains. Based on physiological and phylogenetic characteristics, strain AmH^T is considered to represent a novel species of the genus Nautilia, for which the name Nautilia profundicola sp. nov. is proposed. The type strain is AmH^T (=ATCC BAA-1463^T =DSM 18972^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain AmH^T is AF357197.

Figures showing the specific growth rate of strain AmH^T at varying temperatures, pH and NaCl concentrations are available as supplementary material with the online version of this paper.