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Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex

¹ Laboratorium voor Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium
² Department of Pediatrics, University of Michigan, 1150 W. Medical Centre Drive, 8323 MRSB III, Box 0646, Ann Arbor, MI 48109-0646, USA
³ Department of Biological Sciences, Warwick University, Coventry CV4 7AL, UK
⁴ Department of Pediatrics, University of British Columbia, Vancouver, BC, Canada
⁵ Cardiff School of Biosciences, Cardiff University, Cardiff CF10 3TL, UK

Correspondence
Peter Vandamme
Peter.Vandamme{at}UGent.be

The taxonomic position of five recA gene clusters of Burkholderia cepacia complex (Bcc) isolates was determined using a polyphasic taxonomic approach. The levels of 16S rRNA and recA gene sequence similarity, multilocus sequence typing (MLST) data and the intermediate DNA–DNA binding values demonstrated that these five clusters represented five novel species within the Bcc. Biochemical identification of these species is difficult, as is the case for most Bcc species. However, identification of these novel species can be accomplished through recA gene sequence analysis, MLST and BOX-PCR profiling and by recA RFLP analysis. For diagnostic laboratories, recA gene sequence analysis offers the best combination of accuracy and simplicity. Based on these results, we propose five novel Bcc species, Burkholderia latens sp. nov. (type strain FIRENZE 3^T =LMG 24064^T =CCUG 54555^T), Burkholderia diffusa sp. nov. (type strain AU1075^T =LMG 24065^T =CCUG 54558^T), Burkholderia arboris sp. nov. (type strain ES0263A^T =LMG 24066^T =CCUG 54561^T), Burkholderia seminalis sp. nov. (type strain AU0475^T =LMG 24067^T =CCUG 54564^T) and Burkholderia metallica sp. nov. (type strain AU0553^T =LMG 24068^T =CCUG 54567^T). In the present study, we also demonstrate that Burkholderia ubonensis should be considered a member of the Bcc.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains LMG 24064^T, LMG 24065^T, LMG 24066^T, LMG 24067^T and LMG 24068^T are AM747628–AM747632, and those of the recA sequences of strains LMG 24275, LMG 24066^T, R-24202, LMG 19587, LMG 24271, LMG 24265, R-22599, R-23401, LMG 24067^T, LMG 24065^T, LMG 24270, LMG 19239 and LMG 24064^T are AM748094–AM748105 and AM922300.

Results of DNA–DNA hybridization experiments, HaeIII/MnlI recA gene RFLP patterns and a dendrogram derived from UPGMA linkage of correlation coefficients between BOX-PCR fingerprints are available as supplementary material with the online version of this paper.