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1 Laboratorium voor Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium
2 Department of Pediatrics, University of Michigan, 1150 W. Medical Centre Drive, 8323 MRSB III, Box 0646, Ann Arbor, MI 48109-0646, USA
3 Department of Biological Sciences, Warwick University, Coventry CV4 7AL, UK
4 Department of Pediatrics, University of British Columbia, Vancouver, BC, Canada
5 Cardiff School of Biosciences, Cardiff University, Cardiff CF10 3TL, UK
Correspondence
Peter Vandamme
Peter.Vandamme{at}UGent.be
The taxonomic position of five recA gene clusters of Burkholderia cepacia complex (Bcc) isolates was determined using a polyphasic taxonomic approach. The levels of 16S rRNA and recA gene sequence similarity, multilocus sequence typing (MLST) data and the intermediate DNA–DNA binding values demonstrated that these five clusters represented five novel species within the Bcc. Biochemical identification of these species is difficult, as is the case for most Bcc species. However, identification of these novel species can be accomplished through recA gene sequence analysis, MLST and BOX-PCR profiling and by recA RFLP analysis. For diagnostic laboratories, recA gene sequence analysis offers the best combination of accuracy and simplicity. Based on these results, we propose five novel Bcc species, Burkholderia latens sp. nov. (type strain FIRENZE 3T =LMG 24064T =CCUG 54555T), Burkholderia diffusa sp. nov. (type strain AU1075T =LMG 24065T =CCUG 54558T), Burkholderia arboris sp. nov. (type strain ES0263AT =LMG 24066T =CCUG 54561T), Burkholderia seminalis sp. nov. (type strain AU0475T =LMG 24067T =CCUG 54564T) and Burkholderia metallica sp. nov. (type strain AU0553T =LMG 24068T =CCUG 54567T). In the present study, we also demonstrate that Burkholderia ubonensis should be considered a member of the Bcc.
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains LMG 24064T, LMG 24065T, LMG 24066T, LMG 24067T and LMG 24068T are AM747628–AM747632, and those of the recA sequences of strains LMG 24275, LMG 24066T, R-24202, LMG 19587, LMG 24271, LMG 24265, R-22599, R-23401, LMG 24067T, LMG 24065T, LMG 24270, LMG 19239 and LMG 24064T are AM748094–AM748105 and AM922300.
Results of DNA–DNA hybridization experiments, HaeIII/MnlI recA gene RFLP patterns and a dendrogram derived from UPGMA linkage of correlation coefficients between BOX-PCR fingerprints are available as supplementary material with the online version of this paper.
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