HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Table and Figures Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Vanlaere, E. Articles by Vandamme, P. Search for Related Content PubMed PubMed Citation Articles by Vanlaere, E. Articles by Vandamme, P. Agricola Articles by Vanlaere, E. Articles by Vandamme, P.

Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex

¹ Laboratorium voor Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium
² Department of Pediatrics, University of Michigan, 1150 W. Medical Centre Drive, 8323 MRSB III, Box 0646, Ann Arbor, MI 48109-0646, USA
³ Department of Biological Sciences, Warwick University, Coventry CV4 7AL, UK
⁴ Department of Pediatrics, University of British Columbia, Vancouver, BC, Canada
⁵ Cardiff School of Biosciences, Cardiff University, Cardiff CF10 3TL, UK

Correspondence
Peter Vandamme
Peter.Vandamme{at}UGent.be

The taxonomic position of five recA gene clusters of Burkholderia cepacia complex (Bcc) isolates was determined using a polyphasic taxonomic approach. The levels of 16S rRNA and recA gene sequence similarity, multilocus sequence typing (MLST) data and the intermediate DNA–DNA binding values demonstrated that these five clusters represented five novel species within the Bcc. Biochemical identification of these species is difficult, as is the case for most Bcc species. However, identification of these novel species can be accomplished through recA gene sequence analysis, MLST and BOX-PCR profiling and by recA RFLP analysis. For diagnostic laboratories, recA gene sequence analysis offers the best combination of accuracy and simplicity. Based on these results, we propose five novel Bcc species, Burkholderia latens sp. nov. (type strain FIRENZE 3^T =LMG 24064^T =CCUG 54555^T), Burkholderia diffusa sp. nov. (type strain AU1075^T =LMG 24065^T =CCUG 54558^T), Burkholderia arboris sp. nov. (type strain ES0263A^T =LMG 24066^T =CCUG 54561^T), Burkholderia seminalis sp. nov. (type strain AU0475^T =LMG 24067^T =CCUG 54564^T) and Burkholderia metallica sp. nov. (type strain AU0553^T =LMG 24068^T =CCUG 54567^T). In the present study, we also demonstrate that Burkholderia ubonensis should be considered a member of the Bcc.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains LMG 24064^T, LMG 24065^T, LMG 24066^T, LMG 24067^T and LMG 24068^T are AM747628–AM747632, and those of the recA sequences of strains LMG 24275, LMG 24066^T, R-24202, LMG 19587, LMG 24271, LMG 24265, R-22599, R-23401, LMG 24067^T, LMG 24065^T, LMG 24270, LMG 19239 and LMG 24064^T are AM748094–AM748105 and AM922300.

Results of DNA–DNA hybridization experiments, HaeIII/MnlI recA gene RFLP patterns and a dendrogram derived from UPGMA linkage of correlation coefficients between BOX-PCR fingerprints are available as supplementary material with the online version of this paper.

This article has been cited by other articles:

				J. E. Thwaite, S. Humphrey, M. A. Fox, V. L. Savage, T. R. Laws, D. O. Ulaeto, R. W. Titball, and H. S. Atkins The cationic peptide magainin II is antimicrobial for Burkholderia cepacia-complex strains J. Med. Microbiol., July 1, 2009; 58(7): 923 - 929. [Abstract] [Full Text] [PDF]

				R. J. Malott, E. P. O'Grady, J. Toller, S. Inhulsen, L. Eberl, and P. A. Sokol A Burkholderia cenocepacia Orphan LuxR Homolog Is Involved in Quorum-Sensing Regulation J. Bacteriol., April 15, 2009; 191(8): 2447 - 2460. [Abstract] [Full Text] [PDF]

				H. Rose, A. Baldwin, C. G. Dowson, and E. Mahenthiralingam Biocide susceptibility of the Burkholderia cepacia complex J. Antimicrob. Chemother., March 1, 2009; 63(3): 502 - 510. [Abstract] [Full Text] [PDF]

				J. Foweraker Recent advances in the microbiology of respiratory tract infection in cystic fibrosis Br. Med. Bull., March 1, 2009; 89(1): 93 - 110. [Abstract] [Full Text] [PDF]

				E. Vanlaere, A. Baldwin, D. Gevers, D. Henry, E. De Brandt, J. J. LiPuma, E. Mahenthiralingam, D. P. Speert, C. Dowson, and P. Vandamme Taxon K, a complex within the Burkholderia cepacia complex, comprises at least two novel species, Burkholderia contaminans sp. nov. and Burkholderia lata sp. nov. Int J Syst Evol Microbiol, January 1, 2009; 59(1): 102 - 111. [Abstract] [Full Text] [PDF]

				S. McClean and M. Callaghan Burkholderia cepacia complex: epithelial cell-pathogen confrontations and potential for therapeutic intervention J. Med. Microbiol., January 1, 2009; 58(1): 1 - 12. [Abstract] [Full Text] [PDF]

				M. T. G. Holden, H. M. B. Seth-Smith, L. C. Crossman, M. Sebaihia, S. D. Bentley, A. M. Cerdeno-Tarraga, N. R. Thomson, N. Bason, M. A. Quail, S. Sharp, et al. The Genome of Burkholderia cenocepacia J2315, an Epidemic Pathogen of Cystic Fibrosis Patients J. Bacteriol., January 1, 2009; 191(1): 261 - 277. [Abstract] [Full Text] [PDF]