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Tumebacillus permanentifrigoris gen. nov., sp. nov., an aerobic, spore-forming bacterium isolated from Canadian high Arctic permafrost

¹ Department of Natural Resource Sciences, McGill University, Ste-Anne de Bellevue, Quebec, Canada
² Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, Canada

Correspondence
Lyle G. Whyte
lyle.whyte{at}mcgill.ca

A Gram-positive, aerobic, rod-shaped bacterium (strain Eur1 9.5^T) was isolated from a 9-m-deep permafrost sample from the Canadian high Arctic. Strain Eur1 9.5^T could not be cultivated in liquid medium and grew over the temperature range 5–37 °C; no growth was observed at 42 °C and only slow growth was observed at 5 °C following 1 month of incubation. Eur1 9.5^T grew over the pH range 5.5–8.9 and tolerated NaCl concentrations of 0–0.5 % (w/v). Eur1 9.5^T grew heterotrophically on complex carbon substrates and chemolithoautotrophically on inorganic sulfur compounds, as demonstrated by growth on sodium thiosulfate and sulfite as sole electron donors. Eur1 9.5^T contained iso-C_{15 : 0} as the major cellular fatty acid and menaquinone 7 (MK-7) as the major respiratory quinone. The cell-wall peptidoglycan was of type A1. The DNA G+C content was 53.1 mol%. The 16S rRNA gene sequence of strain Eur1 9.5^T was only distantly related (87 % sequence similarity over 1407 bp) to any recognized bacterial species. Based on physiological and phylogenetic analyses, strain Eur1 9.5^T is suggested to represent a novel species of a new genus, for which the name Tumebacillus permanentifrigoris gen. nov., sp. nov. is proposed. The type strain of Tumebacillus permanentifrigoris is Eur1 9.5^T (=DSM 18773^T =JCM 14557^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Eur1 9.5^T is DQ444975.

A table detailing the cellular fatty acid composition of strain Eur1 9.5^T is available as supplementary material with the online version of this paper.

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				T. D. Niederberger, B. Steven, S. Charvet, B. Barbier, and L. G. Whyte Virgibacillus arcticus sp. nov., a moderately halophilic, endospore-forming bacterium from permafrost in the Canadian high Arctic Int J Syst Evol Microbiol, September 1, 2009; 59(9): 2219 - 2225. [Abstract] [Full Text] [PDF]