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Shinella kummerowiae sp. nov., a symbiotic bacterium isolated from root nodules of the herbal legume Kummerowia stipulacea

¹ Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/College of Biological Sciences, China Agricultural University, Beijing 100094, PR China
² Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 México DF, Mexico

Correspondence
Wen Xin Chen
wenxin_chen{at}263.net

Bacterial strain CCBAU 25048^T was isolated from root nodules of Kummerowia stipulacea grown in Shandong province of China. Cells of the strain were Gram-negative, strictly aerobic, non-spore-forming, motile short rods. Phylogeny of 16S rRNA gene sequences revealed that the strain belonged to the genus Shinella, a member of family Rhizobiaceae. Its closest phylogenetic relatives were Shinella granuli Ch06^T and Shinella zoogloeoides IAM 12669^T, respectively showing 98.3 and 98.9 % 16S rRNA gene sequence similarity. Strain CCBAU 25048^T had DNA–DNA relatedness of 43.5 and 34.8 %, respectively, with S. zoogloeoides JCM 20728^T and S. granuli JCM 13254^T. In addition, in TP-RAPD analysis, different patterns were obtained for these three strains and some rhizobial strains. The nifH, nodC and nodD sequences of CCBAU 25048^T were identical or very similar to those of bean-nodulating Rhizobium tropici strains. Several phenotypic characteristics, including the use of citrate and D-ribose as carbon sources and growth at pH 11.0, as well as the fatty acid composition, could differentiate CCBAU 25048^T from the two defined Shinella species. Therefore, a novel species Shinella kummerowiae sp. nov. is proposed, with strain CCBAU 25048^T (=JCM 14778^T =LMG 24136^T) as the type strain.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain CCBAU 25048^T is EF070131.

A comparison of nifH, nodC and nodD sequences and phylogenetic trees based on these sequences are available as supplementary material with the online version of this paper.

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