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Int J Syst Evol Microbiol 58 (2008), 1398-1403; DOI  10.1099/ijs.0.64681-0
© 2008 International Union of Microbiological Societies

Chromobacterium haemolyticum sp. nov., a strongly haemolytic species

Xiang Y. Han, Faye S. Han and Jonathan Segal

Section of Clinical Microbiology, The University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, USA

Correspondence
Xiang Y. Han
xhan{at}mdanderson.org

A Gram-negative bacterium, strain MDA0585T, isolated from a sputum culture, was characterized by a polyphasic approach. The 16S rRNA gene and a conserved portion of the DNA gyrase A gene were sequenced and analysed phylogenetically. Strain MDA0585T showed the closest relationships with Chromobacterium violaceum ATCC 12472T and Chromobacterium subtsugae PRAA4-1T (96.1 % and 96.3 % 16S rRNA gene sequence similarity, respectively). The cellular fatty acids of strain MDA0585T consisted mainly of C16 : 0, C16 : 1{omega}7c and C16 : 1{omega}6c (summed feature 3) and C18 : 1{omega}7c and C18 : 1{omega}6c (summed feature 8), a profile that was similar to, but distinguishable from, those of C. violaceum ATCC 12472T and C. subtsugae PRAA4-1T. In culture, strain MDA0585T differed from C. violaceum and C. subtsugae in several ways: lack of violet pigmentation, the ability to haemolyse sheep blood, differences in several biochemical reactions and higher resistance to antibiotics. The culture supernatant of strain MDA0585T also caused remarkable haemolysis of human erythrocytes. These results suggest that strain MDA0585T represents a novel species within the genus Chromobacterium, for which the name Chromobacterium haemolyticum sp. nov. is proposed. The type strain is MDA0585T (=CCUG 53230T=JCM 14163T=DSM 19808T).

Abbreviations: RBC, red blood cells

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and gyrA gene sequences of strain MDA0585T are DQ785104 and DQ785105, respectively.

Graphs showing the time course of the growth of strain MDA0585T and Chromobacterium violaceum ATCC 12472T and the lysis of human red blood cells by the culture supernatants are available as a supplementary figure with the online version of this paper. The cellular fatty acid contents of strain MDA0585T and C. violaceum ATCC 12472T are presented in a supplementary table.






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Copyright © 2008 by the International Union of Microbiological Societies.