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Int J Syst Evol Microbiol 58 (2008), 1364-1368; DOI  10.1099/ijs.0.65661-0
© 2008 International Union of Microbiological Societies

Rhizobium miluonense sp. nov., a symbiotic bacterium isolated from Lespedeza root nodules

Chun Tao Gu1, En Tao Wang1,2, Chang Fu Tian1, Tian Xu Han1, Wen Feng Chen1, Xin Hua Sui1 and Wen Xin Chen1

1 Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/Department of Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100094, China
2 Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México DF 11340, Mexico

Correspondence
Wen Xin Chen
wenxin_chen{at}263.net

Seventeen strains of lespedeza rhizobia, a previously defined novel group, were further characterized by comparison with phylogenetically related species using a polyphasic approach, including SDS-PAGE of whole-cell proteins, PCR-based restriction fragment length polymorphism analysis of ribosomal intergenic spacer sequences, determination of DNA G+C contents, DNA–DNA hybridization and analysis of phenotypic features. Based on data obtained in the present and previous studies, a novel species, Rhizobium miluonense sp. nov., is proposed for these strains. The type strain of R. miluonense is CCBAU 41251T (=LMG 24208T=HAMBI 2971T). Strain CCBAU 41251T could form ineffective nodules on Phaseolus vulgaris in addition to its original host Lespedeza chinensis.

Abbreviations: IGS, intergenic spacer; RFLP, restriction fragment length polymorphism

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Rhizobium miluonense CCBAU 41251T is EF061096.

Phenotypic characteristics of strains of Rhizobium miluonense sp. nov. are given in Supplementary Table S1, which is available with the online version of this paper.






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