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Marinobacter guineae sp. nov., a novel moderately halophilic bacterium from an Antarctic environment

Laboratori de Microbiologia, Facultat de Farmacia, Universitat de Barcelona, Av. Juan XXIII s/n, 08028 Barcelona, Spain

Correspondence
Elena Mercadé
mmercade{at}ub.edu

Two Gram-negative, cold-adapted, moderately halophilic, aerobic bacteria, designated strains M3B^T and M3T, were isolated from marine sediment collected from the South Shetland Islands, Antarctica. The organisms were rod-shaped, catalase- and oxidase-positive, and motile by means of polar flagella. These two psychrotolerant strains required Na⁺ and grew at NaCl concentrations of 1–15 % and temperatures between 4 and 42 °C. 16S rRNA gene sequence analysis placed strains M3B^T and M3T within the genus Marinobacter. DNA–DNA hybridization experiments between the Antarctic isolate M3B^T and type strains of phylogenetically related species, namely Marinobacter lipolyticus, Marinobacter flavimaris, Marinobacter sediminum, Marinobacter algicola, Marinobacter maritimus and Marinobacter koreensis, revealed levels of relatedness lower than 32 %. Strain M3T showed 99 % DNA relatedness to strain M3B^T. The DNA G+C contents of M3B^T and M3T were 57.1 and 57.4 mol%, respectively, and their major isoprenoid quinone was ubiquinone-9. Several phenotypic characteristics, together with data on cellular fatty acid composition, served to differentiate strains M3B^T and M3T from strains of related Marinobacter species. On the basis of the polyphasic taxonomic evidence presented in this study, it can be concluded that strains M3B^T and M3T belong to the same genospecies and represent a novel species of the genus Marinobacter, for which the name Marinobacter guineae sp. nov. is proposed. The type strain is M3B^T (=LMG 24048^T=CECT 7243^T).

Fatty acid composition data and DNA–DNA relatedness data for M3B^T and M3T and strains of related species are available with the online version of this paper.