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Pseudomonas panipatensis sp. nov., isolated from an oil-contaminated site

Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110007, India

Correspondence
Rup Lal
duzdel{at}vsnl.com

A Gram-negative, motile, rod-shaped, non-sporulating, aerobic bacterial strain (Esp-1^T) was isolated from oil-contaminated soil of Panipat Oil Refinery, India, and its taxonomic position was determined using a polyphasic approach. Strain Esp-1^T grew in the presence of 2 % NaCl at 30 °C and was characterized chemotaxonomically by having C_{16 : 0} as the major fatty acid followed by C_{17 : 0} cyclo and C_{18 : 1}7c. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Esp-1^T formed a cluster together with Pseudomonas knackmussii DSM 6978^T (98.9 % sequence similarity), Pseudomonas delhiensis MTCC 7601^T (98.5 %), Pseudomonas nitroreducens DSM 14399^T (98.5 %), Pseudomonas citronellolis DSM 50332^T (98.7 %), Pseudomonas multiresinivorans ATCC 700690^T (98.9 %) and Pseudomonas jinjuensis DSM 16612^T (97.8 %). DNA–DNA hybridization values of strain Esp-1^T with P. knackmussii DSM 6978^T, P. delhiensis MTCC 7601^T, P. jinjuensis DSM 16612^T, P. citronellolis DSM 50332^T, P. multiresinivorans ATCC 700690^T and P. nitroreducens DSM 14399^T were 32.9, 30.2, 20.6, 23.4, 23.4 and 20.0 %, respectively. Low levels of DNA–DNA hybridization and phenotypic and chemotaxonomic results are sufficient to delineate strain Esp-1^T from other closely related species of Pseudomonas. Phenotypic and chemotaxonomic data confirm that strain Esp-1^T represents a novel species, for which the name Pseudomonas panipatensis sp. nov. is proposed. The type strain of Pseudomonas panipatensis sp. nov. is Esp-1^T (=MTCC 8990^T=CCM 7469^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Pseudomonas panipatensis Esp-1^T (=MTCC 8990^T=CCM 7469^T) is EF424401.

DNA–DNA hybridization data for strain Esp-1^T and related species are available with the online version of this paper.

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