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Ponticoccus litoralis gen. nov., sp. nov., a marine bacterium in the family Rhodobacteraceae

School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, San 56-1, Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea

Correspondence
Byung Cheol Cho
bccho{at}snu.ac.kr

A Gram-negative, coccoid to rod-shaped, strictly aerobic bacterium, strain CL-GR66^T, was isolated from coastal seawater collected off Korea. The strain grew optimally in the presence of 3–5 % sea salts, at a temperature of 30 °C and at pH 7. The polar lipid profile of strain CL-GR66^T comprised phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, two unidentified aminolipids, an unidentified glycolipid and an unidentified lipid. The major fatty acids were C_{18 : 1}7c (71.4 %), 11-methyl C_{18 : 1}7c (11.3 %), C_{16 : 0} (6.4 %)_, C_{18 : 0} (4.6 %) and C_{12 : 1} 3-OH (3.0 %). Ubiquinone 10 was the major quinone. The DNA G+C content was 67.9 mol%. 16S rRNA gene sequence analysis revealed that strain CL-GR66^T belonged to the Roseobacter clade within the family Rhodobacteraceae. Strain CL-GR66^T was related most closely to the type strain of Marinovum algicola (95.5 % 16S rRNA gene sequence similarity), and sequence similarities between strain CL-GR66^T and other type species of the Roseobacter clade ranged from 91.8 to 95.4 %. Phylogenetic analyses of 16S rRNA gene sequences showed that strain CL-GR66^T was not associated with any known genus in the family Rhodobacteraceae. The distinct phylogenetic position of strain CL-GR66^T, its phylogenetic distance from genera of the family Rhodobacteraceae, together with phenotypic data, suggest that the organism represents a novel species of a new genus, for which the name Ponticoccus litoralis gen. nov., sp. nov. is proposed. The type strain of Ponticoccus litoralis is CL-GR66^T (=KCCM 90028^T =DSM 18986^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain CL-GR66^T is EF211829.

A transmission electron micrograph of negatively stained cells, two-dimensional TLCs of polar lipids, an extended neighbour-joining tree derived from 16S rRNA gene sequences and a table detailing the cellular fatty acid composition of strain CL-GR66^T and phylogenetically related genera in the family Rhodobacteraceae are available as supplementary material with the online version of this paper.