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Int J Syst Evol Microbiol 58 (2008), 1263-1267; DOI  10.1099/ijs.0.65649-0
© 2008 International Union of Microbiological Societies

Kazachstania hellenica sp. nov., a novel ascomycetous yeast from a Botrytis-affected grape must fermentation

Aspasia A. Nisiotou and George-John E. Nychas

Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Technology, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece

Correspondence
George-John E. Nychas
gjn{at}aua.gr

Four ascomycetous yeast strains (D4W13, D9W2, D9W4 and D9W17T) were isolated from Botrytis-affected fermenting grape juice originating from Attica Province, Greece. Phylogenetic analysis of rRNA gene sequences (18S, 26S and 5.8S–ITS) showed that the four strains represent a distinct species within the genus Kazachstania, closely related to Kazachstania zonata NBRC 100504T and Kazachstania gamospora NBRC 11056T. Electrophoretic karyotyping and physiological analysis support the affiliation of the four strains in a novel species for which the name Kazachstania hellenica sp. nov. is proposed, with D9W17T (=CBS 10706T=NBRC 103637T) as the type strain.

Abbreviations: ITS, internal transcribed spacer

The GenBank/EMBL/DDBJ accession numbers for the 26S rRNA, 5.8S–ITS and 18S rRNA gene sequences of strain D9W17T are EF620861, EF620862 and EU075206, respectively; those for the 5.8S–ITS gene sequences of Kazachstania gamospora NBRC 11056T, Kazachstania zonata NBRC 101821, D4W13, D9W4 and D9W2 are EU075198–EU075202, respectively; and those for the 26S rRNA gene sequences of strains D4W13, D9W4 and D9W2 are EU075203–EU075205, respectively.






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