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Saccharopolyspora antimicrobica sp. nov., an actinomycete from soil

Li-Jie Yuan^1,, Yu-Qin Zhang^1,2,, Yan Guan¹, Yu-Zhen Wei¹, Qiu-Ping Li¹, Li-Yan Yu¹, Wen-Jun Li² and Yue-Qin Zhang¹

¹ Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China
² The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China

Correspondence
Wen-Jun Li
wjli{at}ynu.edu.cn
Yue-Qin Zhang
zyq_0525{at}yahoo.com.cn

Three Gram-positive, aerobic, non-motile, non-acid–alcohol-fast strains, designated I05-00051, I05-00074^T and I03-00808, were isolated from different soil samples in Beijing and Sichuan, China. Phylogenetic analysis based on 16S rRNA gene sequences and DNA–DNA hybridization experiments revealed that these three isolates represented the same genospecies. These three strains showed <97.0 % 16S rRNA gene sequence similarity with the type strains of recognized species of the genus Saccharopolyspora, with the exception of Saccharopolyspora hirsuta subsp. hirsuta DSM 43463^T (98.1 % gene sequence similarity) and Saccharopolyspora spinosa DSM 44228^T (98.0 % similarity). Chemotaxonomic data, including meso-diaminopimelic acid as the diagnostic diamino acid, arabinose and galactose as predominant sugars, iso-C_{15 : 0}, iso-C_{16 : 0}, iso-C_{17 : 0} and anteiso-C_{17 : 0} as major fatty acids, MK-9(H₄) as predominant menaquinone and polar lipids dominated by diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol, supported the affiliation of these three organisms to the genus Saccharopolyspora. The genomic DNA G+C contents of the three isolates were 68.2–69.9 mol%. The results of DNA–DNA hybridization experiments among these three isolates and S. hirsuta subsp. hirsuta DSM 43463^T and S. spinosa DSM 44228^T, in combination with chemotaxonomic and physiological data, demonstrated that the three new isolates represent a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora antimicrobica sp. nov. is proposed. The type strain is I05-00074^T (=CCM 7463^T=KCTC 19303^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain I05-00074^T is EF693956.

Tables giving the cellular fatty acid profiles of strains I05-00074^T, I05-00051 and I03-00808 and levels of DNA–DNA relatedness among these three novel strains and reference strains Saccharopolyspora hirsuta subsp. hirsuta DSM 43463^T and Saccharopolyspora spinosa DSM 44228^T are available with the online version of this paper.