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Methylobacterium persicinum sp. nov., Methylobacterium komagatae sp. nov., Methylobacterium brachiatum sp. nov., Methylobacterium tardum sp. nov. and Methylobacterium gregans sp. nov., isolated from freshwater

¹ Microbiological and Analytical Group, Food Research Laboratories, Mitsui Norin Co. Ltd, 223-1, Miyahara, Fujieda, Shizuoka 426-0133, Japan
² Marine Biotechnology Institute Co. Ltd, 3-75-1, Heita, Kamaishi, Iwate 026-0001, Japan
³ Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1, Yayoi 1-chome, Bunkyo-ku, Tokyo 113-0032, Japan

Correspondence
Yuko Kato
ytanaka{at}mitsui-norin.co.jp

Eight strains, 002-165^T, 002-079^T, B0021^T, Hojyo2, RB603B, RB677^T, 002-074^T and RB678, isolated from the environment of food-processing factories in Japan, were characterized using a polyphasic approach. The isolates were Gram-negative, strictly aerobic, pink-pigmented, facultatively methylotrophic, non-spore-forming rods. The chemotaxonomic characteristics of these isolates included the presence of C_{18 : 1}7c as the major cellular fatty acid and ubiquinone Q-10 as the predominant ubiquinone. The DNA G+C content was 67.1–71.1 mol%. Phylogenetic analyses of 16S rRNA and DNA gyrase B subunit (gyrB) nucleotide sequence confirmed that the eight strains belonged to the Methylobacterium clade. Moreover, a DNA–DNA hybridization analysis showed that the eight isolates represented five novel species. On the basis of their phenotypic and phylogenetic distinctiveness, the isolates represent five novel species within the genus Methylobacterium, for which the names Methylobacterium persicinum sp. nov. (type strain 002-165^T =DSM 19562^T =NBRC 103628^T =NCIMB 14378^T), Methylobacterium komagatae sp. nov. (type strain 002-079^T =DSM 19563^T =NBRC 103627^T =NCIMB 14377^T), Methylobacterium brachiatum sp. nov. (type strain B0021^T =DSM 19569^T =NBRC 103629^T =NCIMB 14379^T), Methylobacterium tardum sp. nov. (type strain RB677^T =DSM 19566^T =NBRC 103632^T =NCIMB 14380^T) and Methylobacterium gregans sp. nov. (type strain 002-074^T =DSM 19564^T =NBRC 103626^T =NCIMB 14376^T) are proposed.

A similarity matrix for 16S rRNA and gyrB gene sequences of the isolates and their neighbours is available as supplementary material with the online version of this paper.

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