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Int J Syst Evol Microbiol 58 (2008), 964-969; DOI  10.1099/ijs.0.65490-0
© 2008 International Union of Microbiological Societies

Lutispora thermophila gen. nov., sp. nov., a thermophilic, spore-forming bacterium isolated from a thermophilic methanogenic bioreactor digesting municipal solid wastes

Hatsumi Shiratori1, Hitomi Ohiwa1, Hironori Ikeno1, Shohei Ayame2, Naoaki Kataoka2, Akiko Miya2, Teruhiko Beppu1 and Kenji Ueda1

1 Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa 252-8510, Japan
2 Ebara Research Co. Ltd, 4-2-1 Honfujisawa, Fujisawa 251-8502, Japan

Correspondence
Kenji Ueda
ueda{at}brs.nihon-u.ac.jp

A novel anaerobic, moderately thermophilic, spore-forming, rod-shaped bacterium (strain EBR46T) was isolated from an enrichment culture derived from an anaerobic thermophilic (55 °C) methanogenic bioreactor treating artificial solid wastes. Phylogeny based on 16S rRNA gene sequence analysis placed strain EBR46T within a distinct lineage between Clostridium clusters II and III. The closest recognized relative of strain EBR46T was Gracilibacter thermotolerans DSM 17427T (85.3 % 16S rRNA gene sequence similarity). The DNA G+C content of strain EBR46T was 36.2 mol%. The novel strain grew optimally at 55–58 °C and at pH 7.5–8.0 and was able to grow on peptone, tryptone, Casamino acids, casein hydrolysate, methionine, threonine, tryptophan, cysteine, lysine and serine in the presence of 0.2 % yeast extract. Carbohydrates were not utilized. The main products from tryptone utilization were acetate, iso-butyrate, propionate and iso-valerate. Strain EBR46T produced hydrogen sulfide from cysteine. The major fatty acids were iso-C15 : 0, C14 : 0, C16 : 0 DMA (dimethyl acetal) and iso-C15 : 0 DMA. Based on its unique phylogenetic and physiological features, strain EBR46T is considered to represent a novel species of a new genus, for which the name Lutispora thermophila gen. nov., sp. nov. is proposed. The type strain of the type species is EBR46T (=NBRC 102133T=DSM 19022T).

Abbreviations: DMA, dimethyl acetal

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain EBR46T is AB186360.






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