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Lactobacillus sobrius Konstantinov et al. 2006 is a later synonym of Lactobacillus amylovorus Nakamura 1981

¹ Finnish Food Safety Authority (Evira), Virology, Mustialankatu 3, FIN-00790 Helsinki, Finland
² Department of Basic Veterinary Sciences, Microbiology and Epidemiology, PO Box 66, Agnes Sjöbergin katu 2, FIN-00014 University of Helsinki, Finland
³ Department of Food and Environmental Hygiene, PO Box 66, Agnes Sjöbergin katu 2, FIN-00014 University of Helsinki, Finland

Correspondence
Miia Jakava-Viljanen
miia.jakava-viljanen{at}evira.fi

While studying the taxonomy of six lactic acid bacterium isolates from Finnish porcine intestine and faeces, the taxonomic positions of Lactobacillus sobrius type strain DSM 16698^T and strain AD5 based on comparative 16S rRNA sequence analysis were found to be controversial, as they showed high similarity to Lactobacillus amylovorus strains. Therefore, the taxonomy of these species was addressed in a polyphasic taxonomy study that included, in addition to re-evaluating the 16S rRNA gene sequence and DNA–DNA reassociation results, multilocus sequence analysis (MLSA) of the housekeeping genes encoding the phenylalanyl-tRNA synthase alpha subunit (pheS) and RNA polymerase alpha subunit (rpoA) as well as numerical analysis of HindIII and EcoRI ribotypes. 16S rRNA gene sequence analysis demonstrated a very high similarity between the L. sobrius and L. amylovorus type and reference strains and representative Finnish porcine isolates (99.6–99.9 %). The MLSA data showed the close phylogenetic relationship of these strains; pheS and rpoA gene sequence similarities were 98.5–100 % and 99.6–99.8 %, respectively. Numerical analyses of HindIII/EcoRI ribotypes placed these strains in a single cluster by both enzymes. Finally, the DNA–DNA reassociation experiments revealed high reassociation levels (higher than 79 %) between the strains. These results indicate that DSM 16698^T, AD5 and the related porcine lactobacilli strains from Finland constitute a single species, Lactobacillus amylovorus, and that the name Lactobacillus sobrius should be considered as a later synonym of Lactobacillus amylovorus.

The GenBank/EMBL/DDBJ accession numbers for the pheS and rpoA gene sequences of strains DSM 20531^T, DSM 20532, DSM 16698^T, AD5, LAB7, LAB8, LAB13 and LAB52 are EU000474–EU000489, respectively.

Supplementary phylogenetic trees and a table showing DNA–DNA relatedness among Lactobacillus strains are available with the online version of this paper.