HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Miyazaki, M. Articles by Horikoshi, K. Search for Related Content PubMed PubMed Citation Articles by Miyazaki, M. Articles by Horikoshi, K. Agricola Articles by Miyazaki, M. Articles by Horikoshi, K.

Neptunomonas japonica sp. nov., an Osedax japonicus symbiont-like bacterium isolated from sediment adjacent to sperm whale carcasses off Kagoshima, Japan

¹ Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061, Japan
² Center for Advanced Marine Research, Ocean Research Institute, The University of Tokyo, 1-15-1 Minamidai, Nakano-ku, Tokyo 164-8639, Japan

Correspondence
Masayuki Miyazaki
miyazakim{at}jamstec.go.jp

Novel bacterial species were isolated from sediments adjacent to sperm whale carcasses off Kagoshima, Japan, at a depth of 226–246 m. The isolated strains, JAMM 0745^T, JAMM 1380, JAMM 1475 and JAMM 1610, were Gram-negative, rod-shaped, non-spore-forming and motile by means of a single polar or subterminal flagellum. Phylogenetic analysis based on 16S rRNA gene sequences of the novel isolates indicated a relationship to a symbiotic bacterial clone of the polychaete Osedax japonicus (99.6–99.9 % sequence similarity) and these bacteria were closely related to members of the genus Neptunomonas (95.6–96.0 % similarity) within the class Gammaproteobacteria. The novel strains were able to produce isoprenoid quinone Q-8 as the major quinone component. The predominant fatty acids were C_{16 : 0}, C_{16 : 1} and C_{18 : 1}, with C_{18 : 2} and C_{20 : 2} present in smaller amounts. The DNA G+C contents of the four novel strains were about 43.6–43.8 mol%. Based on the taxonomic differences observed, the four isolated strains appear to represent a novel species of the genus Neptunomonas. The name Neptunomonas japonica sp. nov. (type strain JAMM 0745^T=JCM 14595^T=DSM 18939^T) is proposed for the novel strains.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences acquired during this study are AB288092, AB291226–AB291228 and AB293969–AB293970.

This article has been cited by other articles:

				M. Miyazaki, Y. Nogi, Y. Fujiwara, M. Kawato, T. Nagahama, K. Kubokawa, and K. Horikoshi Amphritea japonica sp. nov. and Amphritea balenae sp. nov., isolated from the sediment adjacent to sperm whale carcasses off Kagoshima, Japan Int J Syst Evol Microbiol, December 1, 2008; 58(12): 2815 - 2820. [Abstract] [Full Text] [PDF]

				M. Miyazaki, Y. Nogi, Y. Fujiwara, and K. Horikoshi Psychromonas japonica sp. nov., Psychromonas aquimarina sp. nov., Psychromonas macrocephali sp. nov. and Psychromonas ossibalaenae sp. nov., psychrotrophic bacteria isolated from sediment adjacent to sperm whale carcasses off Kagoshima, Japan Int J Syst Evol Microbiol, July 1, 2008; 58(7): 1709 - 1714. [Abstract] [Full Text] [PDF]