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Desulfovibrio psychrotolerans sp. nov., a psychrotolerant and moderately alkaliphilic sulfate-reducing deltaproteobacterium from the Himalayas

¹ Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, JNT University, Kukatpally, Hyderabad 500 085, India
² Department of Plant Sciences, School of Life Sciences, University of Hyderabad, PO Central University, Hyderabad 500 046, India

Correspondence
Ch. V. Ramana
r449{at}sify.com
or
sasi449{at}yahoo.ie

A novel sulfate-reducing bacterium (strain JS1^T) was isolated from lake Pangong, a salt-water lake situated in the Himalayas of Leh District, Jammu and Kashmir, India. Cells of strain JS1^T are Gram-negative, vibriod, motile by means of single polar flagellum, contain desulfoviridin, are catalase-positive and can hydrolyse starch. Strain JS1^T grew optimally at pH 8.5–9.0 and at temperatures ranging from 10 to 50 °C (optimum 28–30 °C). Chemo-organoheterotrophy was the only growth mode of strain JS1^T and was possible on a wide range of organic compounds. Sulfate alone but not sulfite, thiosulfate, elemental sulfur, nitrite or nitrate was used as electron acceptor. It has no requirement for NaCl but can tolerate up to 2 % NaCl (w/v). p-Aminobenzoate is a required growth factor. The 16S rRNA gene sequence of the bacterium demonstrated similarity between strain JS1^T and the members of the genus Desulfovibrio. The position of strain JS1^T within the phylogenetic tree indicated that it clustered closely with Desulfovibrio acrylicus DSM 10141^T (89.85 % sequence similarity). On the basis of phenotypic and molecular genetic evidence, it is proposed that strain JS1^T be classified as a novel species of the genus Desulfovibrio of the family Desulfovibrionaceae, with the species name Desulfovibrio psychrotolerans sp. nov. The type strain of the species is JS1^T (=JCM 14597^T=KCTC 5573^T=DSM 19430^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JS1^T is AM418397.

A transmission electron micrograph showing the monopolar flagellum of strain JS1^T is available with the online version of this paper.