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Thalassospira tepidiphila sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from seawater

¹ Laboratory of Applied Microbiology, Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan
² Faculty of Agriculture, Yamagata University, Wakaba-machi 1-23, Tsuruoka 997-8555, Japan
³ Research Center for Oceanography Indonesian Institute of Science, Jl. Pasir Putih I No. 1, Ancol Timur, Jakarta Utara 14430, Indonesia

Correspondence
Kazuya Watanabe
kazuya.watanabe{at}mbio.jp

A Gram-negative, mesophilic bacterial strain, designated 1-1B^T, which degrades polycyclic aromatic hydrocarbons, was isolated from petroleum-contaminated seawater during a bioremediation experiment. A 16S rRNA gene sequence analysis indicated that the isolate was affiliated with the genus Thalassospira in the Alphaproteobacteria; the sequence was found to be most similar to those of Thalassospira profundimaris WP0211^T (99.8 %), Thalassospira xiamenensis M-5^T (98.2 %) and Thalassospira lucentensis DSM 14000^T (98.1 %). However, the levels of DNA–DNA relatedness between strain 1-1B^T and these type strains were 50.7±17.2, 35.7±17.8 and 32.0±21.1 %, respectively. In addition, strain 1-1B^T was found to be distinct from the other described species of the genus Thalassospira in terms of some taxonomically important traits, including DNA G+C content, optimum growth temperature, salinity tolerance, utilization of carbon sources and fatty acid composition. Furthermore, strain 1-1B^T and T. profundimaris were also different with regard to motility and denitrification capacities. On the basis of physiological and DNA–DNA hybridization data, strain 1-1B^T represents a novel species within the genus Thalassospira, for which the name Thalassospira tepidiphila sp. nov. is proposed. The type strain is 1-1B^T (=JCM 14578^T =DSM 18888^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain 1-1B^T is AB265822.