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Int J Syst Evol Microbiol 58 (2008), 692-698; DOI  10.1099/ijs.0.65472-0
© 2008 International Union of Microbiological Societies

Labrys portucalensis sp. nov., a fluorobenzene-degrading bacterium isolated from an industrially contaminated sediment in northern Portugal

Maria F. Carvalho1,{dagger}, Paolo De Marco2,{dagger}, Anouk F. Duque1, Catarina C. Pacheco2, Dick B. Janssen3 and Paula M. L. Castro1

1 Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal
2 IBMC – Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua Campo Alegre, 823, 4150-180 Porto, Portugal
3 Biochemical Laboratory, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands

Correspondence
Paula M. L. Castro
plc{at}esb.ucp.pt

A detailed classification of a novel bacterial strain, designated F11T, capable of degrading fluorobenzene as a sole carbon and energy source, was performed by using a polyphasic approach. This Gram-negative, rod-shaped, non-motile, non-spore-forming, aerobic bacterium was isolated from a sediment sample collected from an industrially contaminated site in northern Portugal. The predominant whole-cell fatty acids were C19 : 0 cyclo {omega}8c, C16 : 0, C18 : 1{omega}7c, C18 : 0, C18 : 0 3-OH and C16 : 0 3-OH. The G+C content of the DNA was 62.9 mol% and the major respiratory quinone was ubiquinone 10 (UQ-10). 16S rRNA gene sequence analysis revealed that strain F11T was a member of the class Alphaproteobacteria and was phylogenetically related to the genus Labrys, having sequence similarities of 95.6 and 93.1 % to the type strains of Labrys monachus and Labrys methylaminiphilus, respectively. DNA–DNA hybridization experiments revealed levels of relatedness of <70 % between strain F11T and the type strains of L. monachus and L. methylaminiphilus (38.6 and 34.1 %, respectively), justifying the classification of strain F11T as representing a novel species of the genus Labrys. The name Labrys portucalensis sp. nov. is proposed for this organism. The type strain is F11T (=LMG 23412T=DSM 17916T).

Abbreviations: FB, fluorobenzene

{dagger}These authors contributed equally to this work.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain F11T is AY362040.






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