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1 Laboratory of Marine Ecosystem and Biogeochemistry, State Oceanic Administration, Hangzhou 310012, People's Republic of China
2 Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, People's Republic of China
3 College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China
4 Institute of Life Sciences, and the Moshe Shilo Minerva Center for Marine Biogeochemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
Correspondence
Min Wu
wumin{at}zju.edu.cn
A Gram-negative, aerobic, neutrophilic, moderately halophilic bacterial strain, HS225T, was isolated from seawater samples around the Zhoushan Archipelago, Zhejiang Province, China. The isolate grew optimally in media with 5.0 % NaCl, at pH 7.0–8.0 and at 25–30 °C. The predominant fatty acids were C16 : 0
9c, C16 : 0, C12 : 0 3-OH and C18 : 1
9c. The genomic DNA G+C content was 59.0 mol%. Based on 16S rRNA gene sequence analysis, the isolate was found to be affiliated to the genus Marinobacter. Strain HS225T exhibited closest phylogenetic affinity to Marinobacter koreensis DD-M3T (98.1 % 16S rRNA gene sequence similarity). DNA–DNA relatedness data and DNA G+C contents as well as physiological and biochemical test results allowed the genotypic and phenotypic differentiation of strain HS225T from closely related species. Therefore, it is proposed that strain HS225T represents a novel species of the genus Marinobacter, for which the name Marinobacter pelagius sp. nov. is proposed. The type strain is HS225T (=CGMCC 1.6775T =JCM 14804T)
A scanning electron micrograph of cells of strain HS225T, phylogenetic trees based on 16S rRNA gene sequences according to the maximum-parsimony and maximum-likelihood methods and a table detailing the fatty acid compositions of Marinobacter strains are available as supplementary material with the online version of this paper.
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